摘要
目的研究吴氏消瘤散对荷瘤S180小鼠肿瘤生长的影响,并观察其免疫调节作用。方法①S180移植瘤模型小鼠随机分为溶剂对照组(n=20)、环磷酰胺组(n=10)及吴氏消瘤散低、中、高剂量组(每组n=10),分别给予相应的干预措施,末次给药后24 h测定各组瘤重及抑瘤率。②将C57BL/6小鼠随机分为溶剂对照组(n=16)、香菇多糖组(n=8)及吴氏消瘤散低、中、高剂量组(每组n=8),分别给予相应的干预措施。以3H-TdR掺入法测定药物对脾T淋巴细胞增殖及NK细胞活性的影响。结果①与溶剂对照组比较,吴氏消瘤散各组小鼠瘤重明显降低,差异具有统计学意义(P<0.01)。②与溶剂对照组比较,吴氏消瘤散各组小鼠能显著增强刀豆蛋白诱导的T淋巴细胞增殖,差异具有统计学意义(P<0.01)。与YAC-1细胞共同培养后,吴氏消瘤散组NK细胞活性明显升高,与溶剂对照组比较差异具有统计学意义(P<0.01)。结论吴氏消瘤散具有抗肿瘤作用,并可促进小鼠脾T淋巴细胞增殖,增强NK细胞活性。
Objective To investigate the effect of "Wushi Xiaoliu Powder" on S180-implanted solid tumor and its regulatory effect on immune system. Methods ① S180 mice were randomly divided into five groups : solvent control group ( n =20 ), cyclophosphamide group ( n = 10 ) and " Wushi Xiaoliu Powder" groups (low, medium and high dose) (n = 10 ), the rats models were treated with cyclophosphamide and " Wushi Xiaoliu Powder", and the tumor weight and tumor inhibition rate were determined 24 h after the last administration. ② C57BL/6 mice were randomly divided into solvent control group (n = 16 ), lentinan group (n =8) and "Wushi Xiaoliu Powder" groups (low, medium and high dose) (n =8 ), each group was treated with corresponding interventions. We determined the spleen T lymphocyte proliferation and the activity of NK cells by 3 H-TdR incorporation method. Results ① Compared with the solvent control group, the tumor weights in these three " Wushi Xiaoliu Powder" groups were significantly reduced (P 〈0.01 ). ② Compared with the solvent control group, the T lymphocyte proliferation induced by Con A in each group were remarkably promoted (P 〈0.01 ). After co-cultured with YAC-I cells, the activity of NK cells were remarkably increased (P 〈0.01). Conclusion "Wushi Xiaoliu Powder" can inhibit S180 solid tumor, promote T lymphocyte proliferation and enhance activity of NK cells.
出处
《上海中医药杂志》
2014年第3期66-69,共4页
Shanghai Journal of Traditional Chinese Medicine
基金
上海市浦东新区科技发展基金资助项目(PKJ2012-Y52)
关键词
吴氏消瘤散
S180移植瘤
肿瘤抑制
免疫调节
"Wushi Xiaoliu Powder"
S180 transplantation tumor
tumor inhibition
immunoregulation