仙鹿消癖胶囊对乳腺增生模型小鼠血管生成因子VEGF、bFGF、MVD表达的影响

Effect of “Xianlu Xiaopi Capsule”on expression of angiogenic factors: VEGF,bFGF and MVD in mice with hyperplasia of mammary gland

目的从血管生成角度探索仙鹿消癖胶囊对乳腺增生症的作用机制。方法将昆明种小白鼠分为空白对照组(15只)、模型对照组(15只)、他莫昔芬组(10只)、仙鹿高剂量组(10只)、仙鹿中剂量组(10只)及仙鹿低剂量组(10只)。除空白对照组外,其余各组小鼠采用腹腔注射苯甲酸雌二醇复制小鼠乳腺增生模型,造模成功后采取相应的干预措施。30天后采用免疫组化PV二步法检测各组小鼠乳腺组织血管生成因子VEGF、bFGF、MVD表达。结果与模型组比较,仙鹿消癖胶囊高、中剂量组均能降低乳腺增生小鼠血管生成因子VEGF、bFGF、MVD的高水平表达,差异有统计学意义(P<0.05,P<0.01);且仙鹿消癖胶囊各剂量组有着明显的量效关系。仙鹿高剂量组与他莫昔芬组比较,VEGF、bFGF、MVD表达差异无统计学意义(P>0.05)。结论仙鹿消癖胶囊通过抑制乳腺增生组织促血管生成因子VEGF、bFGF、MVD的过度表达,抑制乳腺组织中的血管生成。

Objective To explore the effect of ＂Xianlu Xiaopi Capsule＂ on hyperplasia of mammary gland from the view of angiogenesis. Methods Kunming mice were randomly divided into blank control group, model control group, Tamoxifen group, ＂Xianlu Xiaopi Capsule＂ groups （high, medium and low dose）. Except for the blank control group, the mice in others were injected with estradiol benzoate to duplicate the models. After modeling, the corresponding interventions were adopted for each group. The expression of VEGF, bFGF and MVD were detected by the method of two-steps PV. Results Compared with model group, the expression of VEGF, bFGF and MVD in high-dose and medium-dose ＂Xianlu Xiaopi Capsule＂ groups were reduced markedly, with significant difference between them （P 〈0.05,P 〈0.01 ）. There was dose-dependent manner in each ＂Xianlu Xiaopi Capsule＂ group. Compared with Tamoxifen group, no significant difference was found in inhibiting the expression of VEGF, bFGF and MVD in high-dose ＂Xianlu Xiaopi Capsule＂ group （ P 〉 0. 05 ）. Conclusion ＂Xianlu Xiaopi Capsule＂ can inhibit the an^ogenesis in breast tissues by restraining the over-expression of VEGF, bFGF and MVD.