摘要
采用盆栽试验,研究了脂肪族芥子油苷和吲哚族芥子油苷的合成前体甲硫氨酸和色氨酸单独及配合表面活性剂叶面喷施对芥蓝(Brassica alboglabra L.)生长及菜薹中芥子油苷组分和含量的影响。结果表明,各处理对芥蓝生长指标及芥子油苷组分无显著影响,在芥蓝菜薹中均检测到7种脂肪族芥子油苷和4种吲哚族芥子油苷。与喷施清水对照相比,甲硫氨酸、色氨酸单独叶面喷施及表面活性剂辅助的色氨酸叶面喷施,对芥蓝菜薹总芥子油苷含量、总脂肪族芥子油苷含量和总吲哚族芥子油苷含量均无显著影响。表面活性剂辅助下甲硫氨酸叶面喷施处理中菜薹总芥子油苷和总脂肪族芥子油苷含量比清水对照处理分别提高28.5%和31.9%,其中在11种芥子油苷中脂肪族芥子油苷3-丁烯基芥子油苷含量提高幅度最大,达到44.3%,同时表面活性剂辅助下甲硫氨酸叶面喷施显著提高了芥蓝菜薹全硫含量并降低了氮硫比(N/S)。本试验结果表明,有效供给甲硫氨酸能明显促进脂肪族芥子油苷的合成和积累,而辅之以表面活性剂能增进叶面对甲硫氨酸的吸收效果,其影响机理还有待进一步研究。
Chinese kale (Brassica alboglabra L.) is a native endemic variety of Brassica vegetables in China. It is highly rich in anti-cancer glucosinolates (GSs). Recent investigations have shown that methionine and tryptophan were the synthetic precursors of aliphatic and indolyl GSs, respectively. Therefore foliage spray with methionine and tryptophan has been thought to be a potential mode for increasing GSs concentrations in Chinese kale. However, the heavy surface wax layers of Chinese kale affect absorption efficiency of the exogenous amino acids. In this study, pot experiments were carried out to investigate foliage spraying effects of methionine and tryptophan with or without surfactant active agent (SAA) on the growth and GSs composition and concentration in bolting stems of Chinese kale. There was no significant difference in growth indexes and GSs compounds of Chinese kale among the treatments. Eleven individual GSs were separated and identified by liquid chromatography-mass spectrometry (LC-MS) in edible bolting stems of Chinese kale in all the treatments, including seven kinds of aliphatic GSs and four kinds of indolyl GSs. Foliage spraying methionine, tryptophan and tryptophan with SAA had no effect on concentrations of total GSs, total aliphatic GSs and total indolyl GSs. Compared with foliage spray of water, total GSs and total aliphatic GSs concentrations increased by 28.5% and 31.9%, respectively, under foliage spray of methionine with SAA. However, there was no significant difference in total indolyl GSs concen- trations between the treatments. Compared with foliage spring water, foliage spraying methionine combined with SAA significantlyincreased especially the concentration of Gluconapin, increasing on the average by 44.3%. Also foliage spraying methionine com- bined with SAA increased sulfur (S) content in bolting stems of Chinese kale. This in turn decreased nitrogen/sulfur (N/S) ratio, while the changes in S and N/S ratio influenced GSs concentrations in Chinese kale bolting stems. Moreover, the concentrations of individual GSs within a specific GS group (i.e., aliphatic or indolyl) did not change consistently. The above results suggested that methionine absorbed by Chinese kale promoted the synthesis and accumulation of GSs while SAA enhanced the absorption of me- thionine through Chinese kale leaves covered with heavy layers of wax. However, the action mechanism of these substances needed further studies.
出处
《中国生态农业学报》
CAS
CSCD
北大核心
2014年第4期439-446,共8页
Chinese Journal of Eco-Agriculture
基金
国家重点基础研究发展计划(973计划)项目(2007CB109305)
河南省农业科学院优秀青年科技基金项目(2013YQ09)资助