穿心莲内酯联合顺铂对抑制宫颈癌的协同作用

The synergistic anti-cervical cancer effects of andrographolide and cisplatin

目的探讨穿心莲内酯与顺铂联合用药是否具有协同抑制宫颈癌HeLa细胞存活的作用及胱天蛋白酶(Caspase)系统相关的凋亡机制。方法体外培养HeLa细胞株,经穿心莲内酯和(或)顺铂处理后,MTS法检测细胞的存活率,流式细胞仪技术检测细胞凋亡水平变化,蛋白印迹(Western blot)方法检测凋亡相关蛋白Caspase的亚型(Caspase-3、Caspase-8、Caspase-9)和DNA修复酶(PARP)的表达变化。结果穿心莲内酯联合顺铂能够明显抑制HeLa细胞的增殖,其抑制效果明显强于两药单用;穿心莲内酯联合顺铂可以导致HeLa细胞细胞凋亡;两药联用可激活Caspase系统,活化Caspase-3、Caspase-8、Caspase-9,引起PARP蛋白切割。结论穿心莲内酯和顺铂两药联用具有明显的协同作用,能有效抑制HeLa细胞的存活、诱导细胞凋亡,其作用机制可能与诱导凋亡相关信号转导通路Caspase系统活化相关。

Objective To evaluate the synergistic effect of andrographolide and cosplatin on the apoptosis of cer -vical cancer HeLa cells , and to investigate the role of Caspase signal system in this process .Methods After treated by andrographolide and（or） cisplatin, the survival rate of HeLa cells was detected by MTS , while the cellular apoptosis was determined by flow cytometry , and the expression of Caspase -3, -8, -9 and PARP was examined by Western blot . Results As compared with single drug treatment , andrographolide plus cisplatin treatment could significantly reduce the survival of HeLa cells, induce HeLa cellsapoptosis , activate Caspase-3, -8, -9 and thus induce PARP cutting .Con-clusion Andrographolide and cisplatin possess synergistic effects on inhibiting cell proliferation and inducing cell apopto -sis of HeLa cells , which may be related to the activation of the Caspase system .