遵义市规模养猪场大肠杆菌的耐药性检测

Drug Sensitivity Detection of Escherichia coli in Large-scale Pig Farms in Zhunyi City

[目的]掌握遵义市规模养猪场大肠杆菌的耐药情况,为贵州省动物源性细菌监测提供数据。[方法]采集遵义市规模养猪场猪肛门拭子和总排污池新鲜粪便(或污水棉拭子)样品335份,分离出阳性大肠杆菌。采用微量肉汤稀释法对分离的大肠杆菌进行耐药性检测。[结果]分离并鉴定到334株阳性大肠杆菌,分离的大肠杆菌对7种抗菌药物的耐药率依次为:磺胺间甲氧嘧啶钠>土霉素>恩诺沙星>头孢噻呋>氟苯尼考>环丙沙星>庆大霉素,多重耐药性主要集中在4耐、5耐、6耐和7耐。[结论]遵义市规模养猪场大肠杆菌的耐药性严重及多重耐药性正在恶化,且猪场总排污池大肠杆菌对各药物的耐药性皆高于猪肛门拭子。

[Objective] The research aimed to master the drug sensitivity of Escherichia coli in large-scale farms in Zhunyi City and provide data for monitoring animal-derived bacteria in Guizhou Province.[Method] 335 samples of pig anal swab and fresh manure from total sewage pool were collected large-scale pig farms in Zhunyi City to isolate positive E.coli.The drug sensitivity of the isolated E.coli was detected by using broth microdilution method.[Result] 334 strains of positive E.coli were identified.The drug sensitivity rates of the isolated E.coli to 7 kinds of antibacterial drugs were as follows：sulfamonomethoxine Sodium ＞ terramycin ＞ eurofloxacin ＞ ceftiofur ＞ florfenicol ＞ ciprofloxacin ＞ gentamicin.Multiple drug resistance was mainly concentrated in 4、5、6 and 7 resistant.[Conclusion] The drug sensitivity of E.coli was serious in large-scale pig farms in Zhunyi City and the multiple drug sensitivity was worse.The drug sensitivity of E.coli from total sewage pool was higher than that of swine anal swab.