摘要
目的：为了观察土贝母对Ｈｅｐ－２细胞株诱导调亡的机制。方法：应用ＭＴＴ比色法和流式细 胞仪测定细胞周期的变化，并应用电子显微镜技术观察诱导分化后亚细胞结构。结果：土贝母制剂对 Ｈｅｐ－２细胞株有明显的抑制作用，且呈剂量依赖性，流式细胞仪分析，不同的药物浓度和不同的作用时 间凋亡的发生差异均有显著意义，Ｇ１期细胞堆积，Ｓ期细胞减少，Ｇ２／Ｍ期细胞相对增多，亚细胞结构， 细胞线粒体肿胀变性，核固缩。结论：土贝母制剂能阻上Ｇ０／Ｇ１期细胞向Ｓ期转化进程，抑制Ｈｅｐ－２增 殖，促进Ｈｅｐ－２细胞凋亡。
Objective:To investigate the mechanism of the apoptosis of Hep-2 cell line induced by Chinese herb Rhizuma bolbostemmae. Method:The cell proliferation cycle of Hep-2 cell line was measured by methods of thiazole blue colorimetry (MTT) and flow cytometer. The differentiation and subcellular structure of Hep-2 cell line were observed by electronic microscope. Result:There were obvious changes of differentiation of Hep-2 cell line inhibited by Rhizoma bolbostemmae and showed a relation to dose dependence. The results of flow cytometer assay showed that the apoptosis rate of Hep-2 cell line was obviously influnced by the concentration and reaction time of drug. After reaction of Rhizoma bolbostemmae to the Hep--2 cell line,the subcellular structure accumulated in the stage GI,stage S cells decreased and stage G2/M cells increased,and the mitochodria swelled and degenerated,nuclei pycnosized. Conclusion:Chinese herb Rhizoma bolbostemmae have ability to prevent the transition of cell from stage GO/G1 to S,inhibit the Hep--2 cell proliferation and promote the apoptosis of Hep-2 cell line.
出处
《耳鼻咽喉（头颈外科）》
2000年第6期351-354,共4页
Chinese Arch Otolaryngology-Head Neck Surg
