不同海拔梯度暴露下对小鼠脑内ET和NO释放的影响及其与脑水肿的关系

Relationship Between the Release of Intracerebral Endothelin and Nitric Oxide of Mice Exposed to Various Altitude Gradient and High Altitude Brain Edema

目的探讨高原性脑水肿的发生与脑内ET和NO释放量的关系。方法将小鼠置于不同海拔梯度下和高海拔区不同时间暴露下分别测定脑组织中ET和NO含量及脑含水量。结果在急进高原初期脑内ET释放量明显增高 ,NO/ET比值变小 ,进入高海拔区3d后陡然下降 ;NO的释放量在进入高原的前10d都呈明显增高趋势 ,NO/ET比值明显增大 ,进入高原13d后脑内NO释放量开始下降。脑含水量和脑湿干比值以进入高原第9d最高。脑含水量与脑内NO含量之间有显著的相关关系。结论急进高海拔区后脑内NO的过度释放与高原脑水肿有直接关系。

Objective To investigate relationship between the release of intracerebral endothelin(ET) and Nitric oxide(NO) of mice exposed to high altitude regions and pathogenesis of high altitude brain edema.Methods 200 mice exposed to various altitude gradient and various times at high altitude regions were sampling respectively to determine the intracerebral ET and NO content as well as the water content in brain tissue. Results At early stage after rapid entry to high altitude regions the release of intracerebral ET increased obviously, NO and ET ratio was reduced. three days after entry to high altitude regions ET content of intracerebral dropped; the first ten days after entry to high altitude regions NO content of intracerebral increased obviously, NO and ET ratio increased obviously, thirteen days after entry to high altitude regions NO content of intracerebral began to reduce; nine days after entry to high altitude regions, the wet_dry ratio and water content in brain tissue was highest, there is signigicant relationship between brain water content and intracerebral NO content.Conclusions After rapid entry to high altitude regions the hyperrelease of NO in brain may lead to formation of high altitude brain edema.