强直性脊柱炎患者血清白介素9的表达及其意义

Serum Levels and Clinical Significance of Interleukin-9 in Patients with Ankylosing Spondylitis

目的研究强直性脊柱炎(AS)患者不同疾病状态下血清中白介素9(IL-9)的表达水平,探讨其与AS炎症活动的相关性。方法选择2012年10月—2013年4月在河北医科大学第三医院免疫风湿科住院及门诊治疗的AS患者40例,依据Bath强直性脊柱炎活动性指数(BASDAI)分为活动组(n=28)和稳定组(n=12),另选择同时期门诊体检健康者24例为对照组。采用双抗体夹心酶联免疫吸附法(ELISA)检测血清IL-9水平。同时分析AS患者血清IL-9水平与临床指标的相关性。结果稳定组〔(534±80)ng/L〕和活动组〔(691±57)ng/L〕患者血清IL-9水平高于对照组〔(432±56)ng/L〕,活动组患者血清IL-9水平高于稳定组(P<0.05)。AS患者IL-9水平与C反应蛋白(CRP)(r=0.529,P=0.000)、红细胞沉降率(ESR)(r=0.339,P=0.033)、BASDAI(r=0.623,P=0.000)均呈正相关,与病程无相关性(r=0.233,P=0.148)。结论 AS患者血清中IL-9的表达水平显著升高,且与疾病的活动度及临床炎性指标相关,提示IL-9参与并促进了AS的病情发展。

Objective To determine serum levels of interleukin - 9 （ IL - 9 ） in patients with ankylosing spondylitis （AS） in association with the disease activity. Methods A total of 40 outpatients and inpatients with AS undergoing treatments in the Department of Rheumatology of the Third Hospital of Hebei Medical University between October, 2012 and April, 2013, and 24 age - and sex - matched health subjects undergoing regular physical examinations during the same time period were recrui- ted. Patients in the disease group were categorized as an active subgroup （n =28） and a stable subgroup （n = 12）, based on the Bath AS Disease activity index （BASDAI） . Blood was collected from all subjects. Serum levels of IL -9 were measured with a double antibody sandwich enzyme linked immunosorbent assay （ELISA） . The correlation between serum IL - 9 levels and disease activities was analyzed. Results Serum levels of IL - 9 were significantly different between the active disease subgroup （691 ＋ 57 ） rig/L, the stable disease subgroup （534 ~ 80） ng/L, and the control group （432 ~ 56） ng/L （ P 〈 0. 05） . Serum IL - 9 levels in AS patients were positively correlated with C - reactive protein （ r = 0. 529, P = 0. 000）, the erythrocyte sedimentation rate （ r = 0. 339, P = 0. 033 ）, and BASDAI （ r = 0. 623, P = 0. 000）, but not with the course of the disease （ r = 0. 233, P = 0. 148） . Condusion Circulating levels of IL - 9 in patients with AS increase in association with the disease activity and may thereby serve as novel indicator of AS disease progressiion.