野菊花总黄酮对肺癌细胞A549作用研究

Research on the Effect of A549 Lung Cancer Cells Treated by Chrysanthemum Indicum Flavonoids

目的研究野菊花总黄酮对肺癌A549细胞的增殖的影响。方法采用不同浓度梯度野菊花总黄酮作用于肺癌A549细胞,连续作用3d观察细胞形态及密度的变化,并通过MTS比色法检测各种浓度对A549细胞增殖的影响。结果由细胞形态观察及MTS检测可知野菊花总黄酮能有效抑制肺癌A549细胞增殖,并诱导其凋亡,且效果与浓度、时间呈现依赖性。结论野菊花总黄酮对A549细胞具有抑制及诱导凋亡的作用。

Objective To study the influence on A549 lung cancer cell proliferation treated by Chrysanthemum indicum flavonoids. Method After treating A549 cells with different concentrations of Chrysanthemum indieum flavonoids, observing the changes of cell shape and density after continuous actions for 3d, and detecting the influence on A549 lung cancer cell proliferation by MTS assay. Result Chrysanthemum indicum flavonoids could effectively inhibit proliferation and induce apoptosis of A549 lung cancer cell by cell morphological observation and MTS assay in concentration - and time - dependent manners. Conclusion Chrysanthemum indicum flavonoids had the effects of inhibition to A549 lung cancer cells proliferation and inducition of cell apoptosis.