艾灸对类风湿性关节炎大鼠炎症因子和滑膜细胞凋亡的影响

Influences of moxibustion on inflammatory factors and synoviocytes in rats with rheumatoid arthritis

目的探讨艾灸治疗类风湿性关节炎(RA)的作用机理。方法 40只Wistar大鼠随机分为正常组、模型组、艾灸组、针刺组、红外组,每组8只。观察并检测各组大鼠足跖肿胀度,血清白细胞介素1、2(IL-1、IL-2)的含量和关节滑膜组织细胞凋亡抑制基因(Bcl-2)、半胱氨酸蛋白酶(Caspase-3)蛋白表达情况。结果模型大鼠足跖与踝部呈急性炎症性红肿,跖围较正常组明显增粗,血清中IL-1含量升高,IL-2水平降低,Bcl-2、Caspase-3蛋白表达均升高(P<0.05)。经治疗后,艾灸组、针刺组和红外组跖围减小,血清中IL-1含量降低,IL-2水平升高,Bcl-2蛋白表达明显下降(P<0.05),Caspase-3蛋白表达升高(P<0.05)。结论艾灸肾俞穴能抑制RA大鼠继发性足肿胀,抑制血清中致炎因子IL-1的释放,提高免疫调节因子IL-2含量,增强RA大鼠滑膜组织的Caspase-3蛋白表达,降低Bcl-2蛋白表达。

Objective To discuss the mechanism of moxibustion in treating rheumatoid arthritis （RA）. Methods Wistar rats （n = 40 ） were randomly divided into normal group, model group, moxibustion group, acupuncture group and infrared group （each n = 8）. The degree of plantar swelling was observed and content of serum IL-1 and IL-2, and protein expressions of Bcl-2 and Caspase-3 in knee synomium were detected in rats of all groups. Results In model group, plantar and ankle inflammatory swelling was observed, plantar circumference was significantly longer compared with normal group, IL-1 content increased, IL-2 decreased, and expressions of Bcl-2 and Caspase-3 increased （P 〈 0. 05 ）. After treatment, plantar circumference was shorter, IL-1 content decreased, IL-2 increased, expression of Bcl-2 decreased （P 〈 0.05 ）, and expression of Caspase-3 increased （ P 〈 0.05 ） in moxibustion group, acupuncture group and infrared group. Conclusion Moxibustion on Shenshu （BI23） can inhibit secondary plantar swelling and release of IL-1, increase IL-2 content, improve the expression of Caspase-3 and decrease expression of Bcl-2 in knee synomium in RA rats.