充血性心力衰竭患者血浆NO及SOD改变及临床意义

The level of plasm nitric oxide and superoxide dismutase activity and their sigificance in elderly patients with congestive heart failure

目的 为探讨老年充血性心力衰竭 (CHF)患者的血浆一氧化氮 (NO)含量、血液超氧化物歧化酶(SOD)活性及其意义。 方法 采用比色分析法和邻苯三酚自氧化法测定了 40例老年CHF患者的血浆NO浓度及SOD活性。 结果 老年CHF患者的血浆NO [(87 4± 14 2 ) μmol·L-1]显著高于对照组 [(78 1± 2 2 9) μmol·L-1] ,P <0 0 5 ;血液SOD活性 [(178 5± 49 8)U·L-1]显著低于对照组 [(2 76 4± 5 7 3)U·L-1] ,其中心功能Ⅲ～Ⅳ级(按NYHA分级 )患者的血浆NO[(89 8± 9 8) μmol·L-1]显著高于心功能Ⅱ级者 [(79 4± 10 6 ) μmol·L-1] ,P <0 0 1;血液SOD活性 [(15 8 3± 34 6 )U·L-1]显著低于心功能Ⅱ级者 [(188 5± 34 6 )U·L-1] ,P <0 0 1。 结论 老年CHF患者的血浆NO水平增高 ,SOD活性降低 ,且与病情严重程度相关 ,NO及氧自由基在CHF的发生发展中可能起重要作用。

Objective To study the level of plasma nitric oxide(NO)and superoxide dismutase activity and their significance in elderly patients with congestive heart failure(CHF). Methods The plasma NO was measured in 40 elderly patients with CHF by colorimetric analysis and SOD activity by the autoxibation of pyrogallic acid. Results The level of plasma NO was significantly higher in elderly patients with CHF[(87 4±14 2) μmol·L -1 ] than in controls[(78 1±22 9)mol·L -1 ], P <0 05,the SOD activity was significantly lower in CHF patients[(178 5±49 8)U·L -1 ]than in controls[(276 4±57 3)]U·L -1 ], P <0 01,and in witch,the level of plasm NO and SOD activity were associated with NYHA functinoal class. Conclusions There was a increased level of plasma NO and decreased activity of SOD in aged CHF;The NO and oxygen free radicals may play an important role in development of CHF and the level of NO and SOD were associated with the severe degree of CHF.