8-羟基喹啉钐配合物与鲱鱼精DNA的作用研究

Interaction between 8-Hydroxyquiuoline-Sm(Ⅲ) Complex and Herring Sperm DNA

在pH=7.4的Tris-HCl缓冲溶液中,采用荧光光谱法研究表明8-羟基喹啉(HQ)能与稀土金属Sm(III)形成比例为3∶1的配合物。综合运用荧光光谱法、热力学方法、粘度法等分析方法研究了8-羟基喹啉钐配合物与鲱鱼精DNA的作用方式与作用部位,通过EB探针、粘度、磷酸盐、寡聚核苷酸以及Scatchard分析得出了Sm(III)(HQ)3与DNA之间是以部分嵌插以及沟渠作用发生相互作用,且以部分嵌插作用为主。热力学实验表明,Sm(III)(HQ)3能够自发地与DNA发生相互作用,而且这自发过程是焓为主要驱动力,各热力学函数值为ΔrHmΘ=-3.19×105J·mol-1,ΔrGmΘ300.15 K=-9.075×103J·mol-1,ΔrSmΘ=-1.033×103J·mol-1·K-1。Sm(III)(HQ)3能够与DNA以5∶1的比例形成复合物,在300.15和310.15 K形成复合物的结合常数为KΘ300.15 K=1.52×104L·mol-1,KΘ310.15 K=2.48×102L·mol-1。

Using fluorescence spectroscopy, 8-hydroxyquinoline（HQ） and rare earth metal Sm（III） form complexes in the ratio of 3：1 in the buffer solution of Tris-HC1 with pH = 7.4. The interaction mode between 8-hydroxyquinoline-Sm（III） complex and herring sperm DNA were studied by applying analytic methods of fluorescence spectrometry, thermodynamics and viscosity. Through the EB probe, viscosity, phosphate, oligomeric nucleotide and Scatchard analysis, it was concluded that the Sm（III）（HQ）3 and DNA interacted through insertion and ditches and the partial insertion was the key interaction mode. Thermodynamics experiments showed that the Sin（III） （HQ）3 interacted with DNA spontaneously and enthalpy serves as the main driving force during such process. The corresponding thermodynamic function values were △rHm=-3.19×10^5J·mol-1,△rGm300.15K=-9.075×10^3J·mol-1,△rSm=-1.033×10^3J·mol-1·K-1.Sm（Ⅲ）（HQ）3 and DNA formed a complex in a ratio of 5： 1. The combination constant of the complex of 300. 15 and 310. 15 K was K300.15K=1.52×10^4L·mol-1,K310.15K=2.48×10^2L·mol-1.