基质金属蛋白酶及其抑制剂在鼻息肉中表达的临床研究

Matrix metalloproteinases and their inhibitors in the nasal polyps and cytokines

目的探讨基质金属蛋白酶2(MMP-2)及其抑制剂(TIMP-2)在鼻息肉中的表达情况。方法回顾性分析2010年7月至2013年7月40例鼻息肉患者的临床资料,将其作为观察组。另外选择同期体检的健康者40例作为对照组。比较两组MMP-2与TIMP-2的表达情况及探讨二者对细胞因子的影响机制。结果①对照组与观察组MMP-2含量分别为(145.27±25.16)μg/L与(182.11±39.58)μg/L,差异具有显著性(P<0.01);鼻息肉组织中MMP-2阳性细胞免疫组化染色为棕黄色,主要分布于鼻黏膜上皮全层细胞,正常鼻黏膜组织中MMP-2染色非常浅,主要分布于基底细胞层;②对照组与观察组TIMP-2含量分别为(142.11±20.22)μg/L及(159.95±26.86)μg/L,差异无统计学意义(P>0.05);鼻息肉组织中TIMP-2阳性细胞免疫组化染色为棕黄色,均分布于细胞上皮全层细胞,正常鼻黏膜组织中无TIMP-2阳性细胞表达。③观察组MMP-2阳性率显著大于对照组(P<0.01),但两组TIMP-2阳性率差异无统计学意义(P>0.05),且MMP-2与临床分期分型呈正相关性(r>0,P<0.05)。结论 MMP-2与TIMP-2在鼻息肉组织表达失衡,导致鼻上皮细胞结构破坏,可通过对MMP-2/TIMP-2活性的调控来预防与治疗鼻息肉的发生,对预防鼻息肉的临床治疗具有指导意义。

Objective To investigate the matrix metalloproteinase 2 （MMP- 2） and their inhibitors （TIMP -2 ） in nasal polyps and cytokines. Methods A retrospective analysis of the July 2010 to July 2013 in our hospital stay of 40 cases of nasal polyps in patients was performed in clinical data. 40 patients were chosen as a control group at another year in our hospital health examination. The MMP - 2 and TIMP - 2 expression were compared and explored their impact on cytokine mechanisms. Results （i） The MMP -2 levels in control group and observation group were （145.27 ±25.16） μg/L and （182.11 ± 39.58 ）μg/L, respectively. The difference was significant statistical significance （ P 〈0.01 ）. The nasal polyps MMP - 2 positive cells in immunohistochemical staining was brown , mainly in full - thickness nasal epithelial cells. The normal nasal mucosa of MMP - 2 staining is very shallow, mainly in the basal cell layer. （1）The control group and observation group of TIMP - 2 levels were （ 142.11 ± 20.22 ）μg / L and （ 159.95 ± 26.86 ） μg / L, respectively. The difference was not statistically significant （ P 〉 0.05 ）. The nasal polyps TIMP - 2 positive cells by immunohistochemical staining of brown are distributed in all epithelial cell layer of cells, normal nasal mu- cosa without TIMP- 2 positive cells. （3）The MMP -2 positive rate in observation group was significantly higher than the control group （ P 〈 0. 01 ）, but there was no significant difference （ P 〉 0.05 ）. The MMP- 2 and clinical stage Genotyping was positively correlated resistance （ r 〉 0, P 〈 0.05 ）. Conclusion MMP - 2 and TIMP - 2 expression in nasal polyps imbalance, leading to structural damage nasal epithelial cells, can be regulated by MMP - 2/TIMP - 2 activity to prevent the occurrence and treatment of nasal polyps, nasal polyps prevention clinical treatment guidance.