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全血法流式细胞术检测血小板抗体分析 被引量:3

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摘要 目的探讨全血法流式细胞术在检测血小板抗体中的诊断价值。方法选取2011年6月—2013年7月本院收治的特发性血小板减少性紫癜(ITP)患者77例,采用全血法流式细胞术和传统方法分别进行血小板抗体检测,结果进行比较分析。结果采用全血法流式细胞术检测到56例(72.7%)PAIgG阳性,39例(50.6%)血浆抗血小板抗体阳性,总阳性率91.6%;传统方法检测到46例(59.7%)PAIgG阳性,25例(32.5%)血浆抗血小板抗体阳性,总阳性率75.1%。利用全血法流式细胞术检测时,随着血小板减低程度的增加,血小板相关IgG及PAIgM的荧光强度也显著增加,利用全血法流式细胞术检测阳性率高于传统方法检测。结论全血法流式细胞术在检测血小板抗体中极具优势,需血量少、检测结果准确、可重复性好、灵敏度高,对医生提出有效地治疗方案有指导性意义,可作为临床检测的首选方法。
作者 魏丽红
出处 《临床合理用药杂志》 2014年第9期104-105,共2页 Chinese Journal of Clinical Rational Drug Use
关键词 血小板 抗体 诊断
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  • 1李岚,薛俭成.微柱凝胶技术检测513例孕妇IgG抗体效价[J].中国医学工程,2002,10(6):89-90. 被引量:1
  • 2张文超,韦建瑞,蒋作锋,黎庆梅,张锐,郭壮波.流式细胞术检测阿司匹林对冠心病患者血小板活化标志物的影响[J].中国医院药学杂志,2007,27(2):164-167. 被引量:9
  • 3阮淑萍.阿司匹林抵抗[J].江西医药,2007,42(1):85-87. 被引量:2
  • 4Tran HA, Anand SS, Hankey GJ, et al. Aspirin resistance[J]. Thromb Res, 2007, 120(3) : 337 -346.
  • 5Newman PJ. Platelet GPⅡb/Ⅲa: molecular variations and alloantigen[J]. Thromb Haemost, 1991, 66( 1 ) : 111 - 118.
  • 6Michelson AD, Barnard MR, Krueger LA, et al. Circulating monocyte - platelet are a more sensitive marker of in vivo platelet activation than platelet surface P - selectin. Studies in baboons, human coronary intervention, and human acute myocardial infarction [ J ]. Circulation, 2001, 10(4) : 1533 -1537.
  • 7JOHANSSON U, MACEY MG, KENNY D, et al. The role of natural killer T (NKT) cells in immune thrombocytopenia: is strong in vitro NKT cell activity related to the development of remission [J]. Br Journal of Haematol,2005,129 (4) : 564- 565.
  • 8HOUWERZIJL B J, BLOM NR, VAN DER WANT JJ, et al. Ultrastructural study shows morphologic features of apoptosis and paraapoptosis in megakaryocytes from patients with idiopathic thrombocy- topenie purpura[J] . Blood,2004,103 : 500-506.
  • 9KAUSHANSKY K. The molecular mechanism that control thrombopoiesis[J ] . J Cli n Invest, 2005 ,115 (12) :3339 -3347.
  • 10VON DEM BORNE A, FOLMAN C, VAN DEN OUDENRIJN S, et al. The potential role of thrombopoietin in idiopathic thrombocytopenic purpura [ J ] . Blood Rev,2002,16 : 57- 59.

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  • 1胡丽华.临床输血学检验[M].北京:人民卫生出版社,2013.
  • 2Metcalfe P. Platelet antigens and antibody detection [J]. Vox Sanguinis, 2004, 87: $82-$86.
  • 3Rodeghiero F, Stasi R, Gernsheimer TA, et al. Standardization of termi- nology, definitions and outcome criteria in immune thrombocytopenie purpura of adults and children: report from an international working group[ J ]. Blood ,2009,113 ( 11 ) :2386 - 2393.
  • 4Neunert C, Lim W, Crowther M, et al. The American society of hematolo- gy 2011 evidence-based practice guideline for immune thromboeytopenia [ J ], Blood ,2011,117 (16) :4190 - 4207.
  • 5Tran H, Brighton T, Grigg A, et al. A muhi-centre, single-arm, open-la- bel study evaluating the safety and efficacy of fixed dose rituximab in pa- tients with refractory, relapsed or chronic idiopathic thrombocytopenic purpura( R-ITP1000 study) [ J ]. Br J Haematol, 2014,167 (2) : 243 - 251.
  • 6Cines DB, Cuker A, Semple JW. Pathogenesis of immune thrombocytope- nia[J]. Presse Med,2014,43(4Pt2) :e49 -59.
  • 7Moncunill G, Campo JJ, Dobafio C. Quantification of multiple cytokines and chemokines using cytometric bead arrays [ J ]. Methods Mol Biol, 2014,1172:65 - 86.
  • 8Sareban N, Macher S, Drexler C, et al. Platelet antibody analysis by three different tests[ J]. J Clin Lab Anal ,2014, [ Epub ahead of print].
  • 9Badrawy H, Elsayh K1, Zahran AM, et al. Platelet antibodies, activated platelets and serum leptin in childhood immune thrombocytopenic pur- pura[ J]. Acta Haematol,2013,130(4) :312 -318.
  • 10Tomer A, Koziol J, Mcmillan R. Antoimmune thromboeytopenia, flow cytometry determination of platelet-associated antibodies against plate- let specific receptors [ J ]. J Thromb Haemost,2005,3 ( 1 ) : 74 - 78.

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