孤雌生殖海带对草丁膦的敏感性

SENSITIVITY OF PARTHENOGENETIC SPOROPHYTES OF LAMINARIA JAPONICA PHAEOPHYTA TO BASTA

于1999年4—7月，以孤雌生殖海带为材料，运用统计学方法研究其对草丁膦的敏感性，以期得到一定剂量和时间范围内草丁膦对不同长度孤雌生殖海带的半数致死剂量(LD50)。结果表明，孤雌海带在0.5—1.6cm长度范围内，草丁膦的半数致死剂量与海带的长度不相关，而与草丁膦的剂量和作用时间相关。孤雌生殖海带对草丁膦比对氯霉素和潮霉素更为敏感，提示 bar 基因有可能成为海带基因工程更为理想的选择标记基因。

In the experiment, young sporophytes of parthenogenetic L aminaria japonica were divided into three groups according to their total lengths : a. (5.00±0.06)mm, b. (9.50±0.10)mm and c. (16.10±0.13)mm, and were treated with 13 concentrations of basta solution in each group respectively. Basta dose are 0—21.2μg/ml. LD 50 at 36， 48, 60 and 72 hours were calculated. R esults showed that LD 50 of sporophytes of parthenogenetic L. japonic a (0.5—1.6)cm to basta is not correlated with its length. The sporophytes of parthenogenetic L. japonica are more sensitive to basta than to chlorampheni col and hygromycin that have been used as selection pressure in genetic engineer ing of L. japonica. Our observation suggests that bar gene which encodes pho sphinothricin-N-acetyltrensferase can be a more efficient selectable marker ge ne.