鲈鱼群体生化遗传学研究Ⅰ.同工酶的生化遗传分析

BIOCHEMICAL GENETICS OF LATEOLABRAX JAPONICUS POPULATIONⅠ.BIOCHEMICAL GENETIC ANALYSIS OF ISOZYMES

于1994年9月—1995年12月，在青岛近海采集37尾鲈鱼的生化样品，采用淀粉胶和聚丙烯酰胺凝胶2种电泳方法，分析了LDH、MDH、MEP、IDHP、PGM、G3PDH、AK、CK、ADH、SDH、G6PDH、GDH、PGDH、CAT、MPI、EST、SOD等同工酶在鲈鱼眼睛、肌肉、心脏、肝脏、肾脏、鳃、胸鳍等7种组织或器官中的表达情况，并对同工酶表型进行了生化遗传分析，以期为其种质资源保护和开发，以及遗传育种等方面的研究提供基础资料。结果表明，17种同工酶由31个基因座位编码，其中Mep-1、Pgm-1、Sdh、Gdh、Pgdh、Mpi、Est-1、Est-4等8个基因座位为多态。多态座位比例为25 8%。

During Sep. 1994-Dec. 1995, 37 sea bass ( Lateolabrax japonicus) samples were collected from coastal water of Qingdao, Shandong Province. Starch gel electrophoresis and polyacrylamide gel electropho resis were used to detect expression of isozymes in seven organs or tissues: eye , muscle, heart, liver, kidney, gill and pectoral fin. LDH, MDH, MEP, IDHP, PGM, G3PDH, AK, CK were analyzed in Triscitric acid buffer system (TC, pH=6.9); AD H, SDH, G6PDH, GDH, PGDH, CAT and MPI were analyzed in EDTA boric acid Tris buf fe r system (EBT, pH=8.9); SOD and EST in Tris gly buffer system (TG, pH=8.3). Bio chemical genetic analysis showed 17 isozymes were coded by 31 gene loci, 8 of whi ch were found polymorphic. Of the polymorphic loci, Mep-1, Gdh, Pgdh, Est 1 and Est-4 each had two alleles and three phenotypes; Pgm-1, Sdh and Mpi each had thr ee alleles, all six phenotypes of Mpi were observed, but only four of Pgm-1 and three of Sdh were observed, low frequency phenotypes were absent. Mean proportion of polymorphic loci was 25.8%.