江门地区乙型肝炎病毒基因型分布与临床的相关性

Clinical relevance of genotype distribution of hepatitis B virus in Jiangmen region

目的探讨江门地区乙型肝炎病毒（HBV）基因型分布与临床的相关性。方法随机选取本院2013年4～12月本院收治的诊断为慢性乙型肝炎的患者200例，采用实时荧光定量PCR一荧光探针法对HBV的基因型进行检测，并对不同基因型患者的生化学指标及肝功能损害差异进行分析。结果200例慢性乙型肝炎患者的基因型检测结果显示，142例B基因型，43例C基因型，15例BC混合型，不存在其他基因型；肝癌患者和重型肝炎患者中主要为B基因型，分别为90．0％和81．1％，明显高于HBsAg携带者，差异有统计学意义（P〈O．05）：C基因型患者血清丙氨酸氨基转移酶水平明显低于B基因型患者，差异有统计学意义（P〈O．05）。结论江门地区HBV的基因型主要为B型、C型和BC混合型，其中B型基因主要存在于肝癌和严重肝病患者中。

Objective To explore the clinical relevance of genotype distribution of hepatitis B virus （HBV） in Jiangmen region. Methods 200 patients diagnosed with positive chronic hepatitis B admitted to our hospital from April 2013 to December 2013 were randomly selected.The real-time fluorescence quantitative PCR-fluorescence probe method was used to detect the genotypes of HBV.The biochemical indicators of patients with different genotypes were analyzed and the differences in liver function damage of patients with different genotypes were analyzed and explored. Results The genotype detection results of the 200 patients with chronic hepatitis B displayed 142 cases of genotype B,43 cases of genotype C and 15 cases of mixed genotype BC.The main genotype of patients with liver cancer and severe hepatitis was genotype B,accounted for 90.0% and 81.1% respectively,and which were all significantly higher than that of HBsAg carriers,the differences were all statistically significant （P〈O.05）.The serum alanine aminotransferase level of patients with genotype C was significantly lower than that of patients with genotype B,the difference was statistically significant （P〈O.05）. Conclusion The main genotypes of hepatitis B virus in Jiangmen region are type B,type C and mixed type BC,of which genotype B mainly exists in patients with liver cancer and severe liver disease.