蛇床子中佛手柑内酯和异虎耳草素含量的高效液相色谱-二级管阵列检测法同时测定

Determination of Bergamot lactone and Isopimpinellin in Cnidium monnieri by HPLC-DAD

目的建立同时测定蛇床子中佛手柑内酯和异虎耳草素含量的方法。方法采用高效液相色谱（HPLC）法对其含量进行测定，色谱柱为SinoChromC18（250ram×4．6ram，5μm，Elite），流动相为甲醇-0．1％醋酸（55：45），流速1.0ml／min，柱温250C；二极管阵列检测器（DAD）检测器，检测波长310nm。结果佛手柑内酯在0．0315～0．945txg范围内呈良好的线性关系A=2385C～6．666（r=0．9999），异虎耳草素在0．0296—0．888μg范围内呈良好的线性关系A=1711C＋1．203（r=0．9999）；平均回收率分别为99．2％和99．1％，相对标准偏差分别为0．91％和0．67％（n=6）；在常温下8h内稳定性较好。结论HPLC—DAD法简便快速，准确，重现性好，结果可靠，可作为蛇床子的质量控制方法。

Abstract：Objective To establish a determination method of the content of Bergamot lactone and Isopimpinellin in Cnidium monnieri. Methods The content of them in Cnidium monnieri was determined by HPLC. The chromatographic column was Sino- Chrom C18 （250mm ×4.6mm, 5μm, Elite）. The mobile phase was a mixture of Methanol -0.1% Acetic acid aqueous solution （55：45 ）, the flow rate was 1. 0ml/rain, and the column temperature was 25℃. The detector was DAD, and the detection wave- length was 310nm. Results The Bergamot lactone and Isopimpinellin were of good stability in 8h. The calibration curve of them were A =2385C ＋6. 666（ r=0.9999 ） and A = 1711C ＋ 1. 203 （ r =0. 9999 ）, and the linear over the range of 0. 0315 μg ~ 0. 945μg and 0. 0296 μg - 0. 888μg, the average recovery rate were 0.91% and 0.67% （ n = 6 ）. Conclusion The method is convenient, sensitive and accurate. It can be a method for quality control in production of Cnidium monnieri.