神经系统显著表达的丝氨酸/精氨酸富集蛋白1在小鼠大脑发育过程中的表达与分布

Expression of neural salient serine /arginine-rich protein 1( NSSR1) in the development of mouse brain

目的：研究神经系统显著表达的丝氨酸/精氨酸富集蛋白1（NSSR1）在中枢神经系统中的表达情况,比较在小鼠发育过程中NSSR1在中枢神经系统中的表达差异.方法：收集不同胎龄的胎鼠中枢神经系统样本以及不同生长时期的小鼠大脑样品,通过蛋白质印迹法检测NSSR1蛋白在各样品中的表达量,通过免疫组织化学法检测NSSR1在小鼠大脑不同部位发育过程中的表达分布.结果：小鼠中枢神经系统中NSSR1的蛋白表达量在胚胎期9~14 d期间显著增加,其相对表达量从0.186增加至0.445,在出生后则一直维持着比较高水平的表达.免疫组织化学法分析结果显示,在胚胎期12 d的胎鼠中,NSSR1蛋白表达集中在室管膜外侧的套层和边缘层中,在室管膜层中未见表达.在新生小鼠和成年小鼠大脑中NSSR1的表达存在差异：在大脑海马中,成年小鼠NSSR1的表达显著增强,而新生小鼠NSSR1的表达较低;在小脑中,成年大鼠的NSSR1广泛分布在小脑皮质包括浦肯野氏细胞、颗粒细胞等神经元细胞中,而新生鼠的NSSR1主要在浦肯野氏细胞中表达.结论：NSSR1在小鼠大脑中的表达受到发育调控.

Objective： To investigate the expression of neural salient serine/ arginine-rich protein 1 （ NSSR1 ） in the development of mouse brain. Methods： Brain samples were collected from mice with different developmental stages ： 9,12,14 d before birth （E9, El2, El4） and 1 d, 3 weeks and 3 months after birth. The expression ofNSSR1 in mouse brain at different developmental stages was detected by Western blot and the distribution of NSSR1 was analyzed by immunohistochemical staining. The expression and distrubution of NSSR1 in mouse brain were compared among embryos, neonatal and adult animals. Results： During embryogenesis, the expression of NSSR1 proteins increases significantly from 0. 186 （ E9 ） to 0. 445 （E14） and reached a high level after birth. Immunohistochemical analysis showed that in El2 embyos, NSSR1 was specifically distributed in the marginal and mantle layers. The expression of NSSR1 in hippocampus was very low in neonatal animals but stonger in adults. In cerebellar cortex, NSSR1 was widely expressed in purkinje and granule cells of adult animals, but mainly expressed in Purkinje cells in neonates. is regulated by the development of mouse brain Conclusion： The expression of NSSR1 and presents dynamic changes.