介入方法兔急性脑梗死模型的制作及CT灌注成像评价

Establishment of rabbit with acute cerebral infarction by intervention and evaluation of computed tomographic perfusion imaging

目的 探讨介入方法兔脑梗死模型的制作及CT灌注成像诊断价值.方法 选择新西兰兔12只,经股动脉插管于颅内动脉注入自体血栓,术后2及6h行脑血管造影及CT灌注成像（CTP）检查.结果 12只新西兰兔均成功栓塞颅内动脉,造模成功率为100％;造影显示大脑中、前动脉分支减少.术后2h行CTP示脑血流量（CBF）、梗死区脑容积（CBV）数值下降,平均通过时间（MTT）和峰值时间（TTP）延长;对比分析梗死区、健侧区CTP各参数值差异有统计学意义（P＜0.01）.6h行CTP示栓塞区CBF、CBV参数值较2h降低,MTT、TTP值较2h延长.结论 介入法兔脑梗死制模方法操作简单、成功率高、创伤小,为脑梗死动物实验理想的造模方式;CT灌注成像是诊断急性脑梗死的有效检查方法.

Objective To discuss the establishment of acute cerebral infarction model in rabbits and evaluate the value of computed tomographie perfusion （CTP） imaging. Methods A total of 12 healthy adult New Zealand white rabbits were used. The model was established through femoral artery puncture and injecting autoblood clot into internal carotid artery through an inserted micro-catheter to occlude the artery. Digital subtraction angiography （ DSA ） cerebral angiography and CTP imaging were performed at 2 and 6 hours post-embolization. Results Super-selective catheterization of internal carotid artery and an injection of auto- blood clots were successfully accomplished in all rabbits and the success rate was 100%. DSA showed that middle cerebral artery and anterior cerebral artery narrowed. At 2 hours post-embolism, CTP showed cerebral blood flow（CBF） and cerebral blood volume（CBV） decreased and mean transit time（MTT） and time to peak （TTP）increased in cerebral ischemia area. Compared with the contralateral mirror area, CBF and CBV decreased in infarct area and MTF and TTP elongated with significant statistical difference at 2 and 6 hours （ P 〈 0. 01 ） . Conclusion The model of acute cerebral ischemia may be effectively established in rabbits. This micro-invasive technique is easily manipulated with a high success rate. And CTP is an effective way of diagnosing acute cerebral infarction.