一株鸡副粘病毒F基因重要片段的扩增、克隆与分析

Nucleotide Sequence Analysis of Main Function Domain of F Gene of An Unassigned Chicken Paramyxovirus Isolate

应用RT_PCR技术对新城疫病毒 (NDV)Y98株的F基因重要功能区片段扩增、克隆后进行序列测定 ,并与多株已报道的NDV参考株相应片段进行序列比较 ,经遗传基因进行化树分析后 。

A new Avian Paramyxovirus (APMV) isolate was obtained in Jiangsu Province in 1998,which named isolate Y98.Part of the fusion gene of isolate 98 were amplified by RT_PCR and sequenced.A phylogenetic tree based on obtained sequences of reference NDV strains was constructed and showed that isolate Y98 was similar to the genotype VII NDV virus,a novel genotype NDV,which caused numbers outbreaks in recent in Western Europe and Far East.It's the first report of the genotype VII NDV isolated in mainland of china.