糖肾平对糖尿病肾病大鼠足细胞nephrin与CD_2AP蛋白及其mRNA表达的影响

Effect of Tangshenping Capsule on Expression of Nephrin and CD_2AP in Podocytes of Early Diabetic Nephropathy Rats

目的:观察糖肾平对糖尿病肾病(DN)大鼠足细胞裂孔隔膜蛋白分子nephrin和CD2AP表达的影响,探讨其防治DN的作用机制。方法:雄性Wistar大鼠74只,按体重随机分组为正常组10只,造模组64只。造模组大鼠腹腔一次性注射链脲佐菌素(STZ)(45 mg/kg),72 h后检测大鼠血糖和尿糖,以血糖≥16.7 mmol/L、尿糖(++++)者为DN大鼠造模成功。模型成功的大鼠按体重随机分为模型组、厄贝沙坦组、糖肾平小、大剂量组,各组分别干预14周。于第14周处死大鼠,无菌取肾,采用原位杂交和免疫组化技术观察肾组织足细胞裂孔隔膜蛋白nephrin和CD2AP蛋白及其mRNA的表达水平。结果:模型组与正常组相比,nephrin和CD2AP蛋白及mRNA表达水平明显下调(P<0.01);与模型组相比,厄贝沙坦、糖肾平干预后糖尿病大鼠肾小球足细胞nephrin和CD2AP蛋白及mRNA水平表达明显增加(P<0.05)。结论:糖肾平可能通过维持足细胞裂孔隔膜蛋白nephrin和CD2AP的稳定性,从而发挥对DN肾脏的保护作用。

Objective：To observe the effects of Tang Shen Ping Capsule on the expression of Nephrin and CD2 AP in podocytes of diabetic nephropathy （DN） rats. Methods： Seventy - four male Wistar rats were randomly divided into the normal group （n = 10） and the model group（ n = 64）. The model of diabetes was induced by intraper -itoneal injection of streptozotocin （STZ 45 mg/kg）. Then The diabetic rats were randomly divided into 4 groups：the model group, the irbesartan group, the small dose tangshenping capsule group and the high dose tangshenping capsule group. All the rats were gavaged for 14 weeks. The protein and mRNA levels of nephrin and CD2 AP in the kidney tissues were also determined by immunohistochemistry and in 1 situ hybridization. Then observed under light microscope. RT - PCR detection of kiney nephrin mRNA. Results： After tangshenping capsule intervention, the levels of Nephrin and CD2 AP also markedly increased （P 〈 0.01 ）. Conclusion ：Tangshenping capsule might protect kidney against STZ induced injury via increasing the expression of nephrin and CD2AP in podocytes.