抗传染性法氏囊病病毒单克隆抗体特性的研究

Study on the Characteristics of monoclonal antibody against infectious bursal disease virus

试验采用琼脂免疫双扩散 ,酶联免疫吸附试验 ( EL ISA) ,病毒中和试验和 Western-blotting试验对 13株单克隆抗体 ( Monoclonal Antibody,Mc Ab)的特性进行了研究。试验结果表明 ,其中 9株 Mc Abs为 Ig G类 ,4株为 Ig M类 ;这些 Mc Abs仅与抗传染性法氏囊病病毒( Infectious Bursal Disease Virus,IBDV)发生特异结合 ,而与新城疫病毒 ( Newcastle DiseaseVirus,NDV)、传染性支气管炎病毒 ( Infectious Bronchitis Virus,IBV)和马立克氏病病毒 ( Marek'sDisease Virus,MDV)均不发生交叉反应 ,有 5株 Mc Abs具有中和 IBDV的活性 ,这 5株有中和活性的 Mc Abs识别的抗原位点在 IBDV的 VP2

Thirteen strains of monoclonal antibodies (McAbs) against infectious bursal disease virus (IBDV) were obtained by using hybridoma technique and their characteristics were studied by double immunodiffusion,enzyme linked immunosorbent assay (ELISA),virus neutralization test (VNT) and Western blotting assay (WBA).The result showed that nine of the thirteen McAbs belonged to IgG class and four of them belonged to IgM class.No crossreactions were detected between the McAbs and Newscastle disease virus (NDV),infectious bronchitis virus (IBV) and Marek's disease virus (MDV).All of McAbs were positively specific reactive with IBDV and five of them could neutralize viral infectivity.Their recognized epitopes of the neutralizing McAbs were all presented on VP 2 of the IBDV.$$$$