Zyflamend对实验性口腔癌抑制作用的研究

The inhibition effects of Zyflamend on DMBA-induced oral carcinogenesis

目的:观察抗炎中药混合制剂Zyflamend(ZF)对DMBA诱发的金黄地鼠口腔癌的抑制作用。方法:116只金黄地鼠,10只设为阴性对照,其余用0.5%的DMBA石蜡油涂于地鼠左侧颊囊,3次/周,共涂6周。从第7周开始,将动物分为3组。给低浓度组(n=33)地鼠左侧颊囊局部涂抹1∶1石蜡油稀释的ZF,高浓度组(n=33)涂ZF原液,3次/周,连续18周,阳性对照组(n=40)不给药。第24周末处死动物,处死前2 h腹腔注射50 mg/kg的5-溴脱氧尿嘧啶(BrdU)。取左侧颊囊进行组织病理学观察和BrdU免疫组化染色。结果:未防治组低、高浓度ZF组,肉眼观察肿瘤发生率分别为86.7%、53.6%(P<0.01)和50.0%(P<0.01);组织病理学观察鳞状细胞癌的发病率分别为86.7%、78.6%(P>0.05)和64.3%(P<0.05)。ZF高浓度组的病变中组织,BrdU的增殖指数显著降低(P<0.05)。结论:Zyflamend能够降低DMBA诱导金黄地鼠口腔癌的发生率,其机制可能与抑制细胞增殖有关。

Objective：To study the effects of an unique anti-inflammatory herbal preparation Zyflamend（ZF）on 7,12-dimethyl-benz anthracene （DMBA）-induced oral carcinogenesis in golden Syrian hamsters.Methods：10 hamsters were used as the blank controls.106 hamsters were treated topically on the left cheek pouch with 0.5% DMBA solution,3 times per week for 6 weeks. Then the hamsters in positive control group（n =40）were not treated afterwards,in the other 2 groups were treated by topically ap-plication of ZF of original solution（high dose,n =33）and 1 ∶1 dilution（low dose,n =33）respectively.Hamsters were sacrificed at the 24th week and tissue samples of the left cheek pouch were obtained for the examination of tumor incidence,histopathology and BrdU immunohistochemisty.Results：Gross inspection showed that the incidences of the tumor in positive,low dose and high dose groups were 86.7%,53.6%（P 〈0.01 ）and 50.0%（P 〈0.01 ）,histopathological examination showed that the tumor incidences were 86.7%,78.6% and 64.3%,respectively（P 〈0.05）.High dose of ZF significantly decreased the incidence of hyperplasia , dysplasia and cancer（P 〈0.05）;decreased BrdU-labeling index（P 〈0.05）.Conclusion：Zyflamend may inhibit DMBA induced oral carcinogenesis and such prevention may be related to the effect of the suppression of cell proliferation.