HIV 感染者唾液 CMV、HHV-6、HHV-7和 HHV-8巢式 PCR 检测分析

Nested-PCR detection of cytomegalovirus and herpesviruses 6,7 and 8 in saliva from human immunodeficiency virus-infected subjects

巢式PCR技术检测245例HIV感染者唾液CMV、HHV-6、HHV-7和HHV-8 DNA,检出率分别为34.7%、83.3%、70.2%和14.3%;30例健康对照组分别为10.0%、56.7%、70.0%和0%,2组比较,P<0.01。使用HAART(n=100)与未使用HAART的HIV患者(n=145)比较,4种HHV唾液检出率无差异(P>0.05)。受检者都存在CMV、HHV-6、HHV-7和HHV-8多重感染。

CMV,HHV-6,HHV-7 and HHV-8 DNA in unstimulated whole saliva from 245 HIV-infected subjects and 30 healthy controls were examined by nested-PCR assays.Prevalence of CMV,HHV-6,HHV-7 and HHV-8 in saliva of HIV-infected subjects was 34.7%, 83.3%,70.2% and 14.3% respectively,that of the controls 10.0%,56.7%,70.0% and 0% respectively（between 2 groups,P 〈0.01）.There was no difference of detection rates of the four HHVs in saliva between HIV patients with HAART（n =100）and non-HAART （n =145）（P 〉0.05）.Multi-infection was observed in all subject.