广西2009～2011年鸡传染性支气管炎病毒分离株的基因分型及血清分型

Genotypes and Serotypes of Avian Infectious Bronchitis Viruses Isolated during 2009-2011 in Guangxi,China

为了解广西地区1985年以来传染性支气管炎病毒（Infectious bronchitis virus，IBV）的流行情况及跟踪其基因和抗原的进化，通过病毒基因扩增与序列测定技术以及鸡胚气管环中和试验，分别对2009～2011年间分离的28个广西地方IBV毒株进行基因分型和血清分型。毒株基因序列测定与比较分析的结果表明，28个IBV分离株在N基因的进化关系可分成3个主要的基因群，其中大多数毒株属于第Ⅲ群，而之前1985～2008年的流行毒株则属于第Ⅳ群和第Ⅱ群；病毒中和试验的结果表明，28个IBV分离株分属于6个不同的血清型，其中血清1、2和3型共占71．4％，与现用疫苗株血清型一致的毒株共有11个（占39．3％）。结合本课题组之前的研究结果进一步的分析还发现，广西不同地区存在不同血清型的流行且不同时期流行的血清型及其所占比例也不尽相同。本研究为研发和应用包含多个优势血清型的多价灭活油苗奠定了很好的基础。

In order to investigate the prevalence and track genetic and antigenic evolutions of infectious bronchitis virus （IBV） and their prevalence in Guangxi, China since 1985, gene amplification and sequencing and virus neutralization （VN） test on chicken embryo tracheal organ cultures were used in genotyping and serotyping of 28 IBV isolates during 2009-2011 in Guangxi. The results of N gene sequencing and comparison showed that the 28 isolates and reference strains were classified into three groups, and most isolates belonged to group III, while the isolates in 1985-2008 belonged to groups IV and II. The data of VN test indicated that the 28 isolates belonged to 6 serotypes; among them, 71.4% belonged to serotypes 1, 2, and 3, and 11 （39.3 %） shared the same serotype with the current vaccine strains. Given the data of our previous study, it is found that prevalent serotypes and their proportions varied in different areas of Guangxi and during different periods. These data lay a good foundation for developing an oil-emulsified inactivated polyvalent vaccine containing local dominant serotypes for the effective prevention and control of infectious bronchitis.