2型糖尿病对小鼠骨代谢的影响

Effect of type 2 diabetes mellitus on bone metabolism: an in vivo study

目的观察2型糖尿病小鼠的骨代谢及骨微结构的特点。方法采用雄性KK/Upj-Ay/J小鼠(自发性2型糖尿病模型小鼠)10只作为实验组,同时选用10只雄性C57BL/6小鼠作为对照组。两组小鼠均给予常规饲料喂养,确认KK/Upj-Ay/J小鼠发病,继续饲养12周后处死所有小鼠,测定血清骨碱性磷酸酶(BALP)及抗酒石酸酸性磷酸酶(TRAP)活性,并运用MicroCT分析小鼠胫骨微结构定量参数。结果与对照组相比,2型糖尿病小鼠血清BALP活性明显下降(分别为对照组:0.029±0.003μU/min,T2DM组:0.014±0.003μU/min,P<0.05),血清TRAP活性明显升高(分别为对照组:0.513±0.034 U/L,T2DM组:0.701±0.054 U/L,P<0.05),胫骨平台处骨密度明显下降(对照组:810.000±21.000 mg/cm3,T2DM组:709.000±18.000 mg/cm3,P<0.05)。结论 2型糖尿病小鼠的骨吸收加快而骨形成不足,导致其骨量下降及骨折风险增大。

Objective To observe the effect of type 2 diabetes mellitus （T2DM） on bone metabolism and bone microstructure in mice.Methods Ten male KK/Upj-Ay/J mice, a mouse model of T2DM, were selected as experimental group, and 10 male C57BL/6 mice were selected as control group.The mice in both groups were fed with routine fodder.After the confirmation of T2DM in KK/Upj-Ay/J mice, all mice were fed for another 12 weeks and then executed.The activity of serum bone alkaline phosphatase （BALP） and tartrate-resistant acid phosphatase （ TRAP） were detected.The parameters of the tibia were analyzed using micro-CT.Results Compared with those in control group, the activity of serum BALP in T2DM mice decreased significantly （control group：0.029 ±0.003 μU/min; T2DM group： 0.014 ±0.003 μU/min, P〈0.05）, while the activity of serum TRAP increased significantly （control group：0.513 ±0.034 U/L;T2DM group：0.701 ±0.054 U/L, P〈0.05）.The bone mineral density of the tibial plateau decreased significantly in T2DM group compared with that in control group （ control group：810.000 ±21.000 mg/cm3; T2DM group：709.000 ±18.000 mg/cm3, P〈0.05）.Conclusion The bone resorption in mice with T2DM accelerates while the bone formation is deficient, resulting in osteopenia and increased risk of bone fracture.