蕹菜Cd-PSC根际土壤酶和微生物特征的研究

Characterization of Soil Enzyme and Microorganism of Ipomoea aquatica Forssk. Cd-PSC Rhizosphere

[目的]为了研究在2种不同镉浓度的农田上蕹菜Cd-PSC和non-Cd-PSC 2个品种根际土壤酶和微生物的特征。[方法]采用自制根箱试验。[结果]蕹菜根际土壤5种酶(脲酶、转化酶、酸碱磷酸酶、蛋白酶)活性和三类微生物(细菌、真菌、放线菌)数量都显著大于非根际土壤(P<0.01)。在同一土壤上,蕹菜品种间酶活性和微生物数量均存在基因型差异(P<0.05);在2种土壤上,脲酶、转化酶、蛋白酶在2个品种间表现不一致,而酸碱磷酸酶则一直表现为Cd-PSC的根际酶活性小于non-Cd-PSC的活性。[结论]Cd-PSC的根系细菌、放线菌的数量显著小于non-Cd-PSC的根系土壤中的数量(P<0.01),而真菌数量显著大于non-Cd-PSC的数量(P<0.05)。

[ Objective ] The research aimed to research the characterization of soil enzyme and microorganism of Ipomoea aquatica Forssk. Cd- PSC and non-Cd-PSC rhizosphere in different Cd concentrations soil. [ Method] A root box experiment was carried out. [ Result] Five soil en- zymatic activities （ urease, invertase, ale and alkalin phosphatase, proteolytic） and the number of three soil microorganisms （bacteria, fungi, actinomyces ） of Ipomoea aquatica Forssk. rhizosphere were dramatically higher than those of non-rhizosphere（ P 〈 0.01 ）. In the same soil, the activity of soil enzymes and the number of soil microorganisms had some genetic variation（ P 〈 O. 05 ）. In different soil, the activities of ure- ase, invertase and proteolytie were different between the two cultivars, but the activities of aic and alkalin phosphatases were the same. That is, the enzymatic activities of lpornoea aquatica Forssk. Cd-PSC rhizosphere was lower than that of non-Cd-PSC. [ Conclusion ] The number of bacteria and actinomyees of Cd-PSC rhizosphere was dramatically lower than that of non-Cd-PSC, but the fungi number was dramatically higher than that of non-Cd-PSC.