摘要
目的分析我院产KPC酶肺炎克雷伯菌的耐药性及基因型。方法收集2012年2月至2013年5月期间分离自我院临床科室的对亚胺培南耐药的肺炎克雷伯菌9株,采用VITEK 2 Compact全自动微生物鉴定系统进行细菌鉴定及药敏试验,采用改良Hodges试验对KPC酶表型进行检测,采用PCR法对菌株的KPC酶基因型进行检测。结果所分离的9株肺炎克雷伯菌对所选用的19种抗生素均100.0%耐药,有7株改良Hodges试验呈现阳性结果,阳性率77.8%。9株肺炎克雷伯菌均扩增出KPC目的基因条带,且均为KPC-2型基因。结论产KPC酶肺炎克雷伯菌耐药形势严峻,合理使用抗菌药物,加强感染者的消毒与隔离,是控制此类细菌感染的有效措施。
Objective To analyze the drug resistance and genotype of KlebsieUapneumoniae which producing Klebsiellapneumoniae earbapenemase（KPC）. Methods 9 strains Klebsiellapneumoniae resistant to imipenem were isolated from February 2012 to May 2013 in our hospital. Identification of bacteria and drug sensitive test were done by VITEK 2 Compact automatic microbiological assay system. The phenotype of KPC was detected by modified Hodges test and the genotype of KPC was detected by PCR method. Results The isolated 9 strains were all resistant to 19 kinds of antibiotics. There were 7 strains showen positive by Hodges test, and the positive rate was 77.8%. The 9 strains Klebsiellapneumoniae were all had KPC gene, and were all KPC-2 gene. Conclusion The resistance condition of Klebsiella pneumoniae producing KPC is austerity. Using antibacterials rationally, reinforcing disinfection and isolation of infected person are the utility measures for controlling this kind of bacterial infections.
出处
《实用检验医师杂志》
2013年第4期229-232,共4页
Chinese Journal of Clinical Pathologist
关键词
肺炎克雷伯菌
肺炎克雷伯菌碳青霉烯酶
耐药性
抗生素
Klebsiellapneumoniae
Klebsiella pneumoniae carbapenemase
Drug resistance
Antibiotics
