溴氰菊酯对烟草K326抗氧化酶活性和MDA含量的影响

Effects of deltamethrin insecticide on the content of MDA and activities of antioxidant enzymes in tobacco K326

[背景]目前,在农林业害虫治理中,化学防治法占有主导地位.然而,在农药的实际应用中,人们更多关注的是其杀虫效果,忽略了其对植物影响的研究.[方法]将生产中常用的杀虫剂溴氰菊酯用丙酮配制成500、1000和1500倍液,当温室中培育的烟草幼苗长至9叶1芯时,在第9片叶上轻轻涂抹杀虫剂（100 μL）,用硫代巴比妥酸法、氮蓝四唑和愈创木酚法等,测定药后24 h烟草幼苗的丙二醛（MDA）含量和抗氧化酶[超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、抗坏血酸过氧化物酶（APX）、谷胱甘肽过氧化物酶（GPX）]活性.[结果]溴氰菊酯可导致烟草幼苗MDA含量下降,抗氧化酶SOD、CAT、APX、GPX活性降低,且随着药剂浓度增大,下降幅度升高.其中,1500和1000倍液处理结果与对照差异显著,500倍液处理结果与对照差异极显著.[结论与意义]溴氰菊酯500 ～ 1500倍液对烟草具有生态安全性,不会造成其氧化胁迫反应.

[ Background ] Nowadays, chemical control plays an important role in the agroforestry production. The effects of insecti- cide on insects have been heavily studied, but their effects on plants have often been ignored. [ Method ] Deltamethrin, an insecti- cide often used in agriculture, was diluted with acetone to 500 times, 1000 times and 1500 times. When the tobacco seedlings at nine-leave stage which were raised under greenhouse condition, the contents of MDA and activities of antioxidant enzymes [ superox- ide dismutase （SOD）, catalase （CAT）, ascorbate peroxidase （APX）, glutathione peroxidase （GPX）] were tested after treated with 100 tzL deltamethrin. [ Result] The results showed that deltamethrin led to lower MDA content, and the activities of SOD, CAT, APX, and GPX in tobacco leaves decreased. And it was obvious that the higher the concentration, the greater the decline. [ Conclusion and significance]The deltamethrin did not cause the oxidative stress response of tobacco seedlings. So it was ecological safety within the concentration range of 500 - 1500 times.