基于正交设计法的利福平明胶微球制备工艺优化

Orthogonal Optimization for Preparation of Rifampicin Gelatin Microspheres

采用正交试验及单因素对利福平明胶微球的最佳工艺条件(包括表面活性剂类型、乳化剂浓度、乳化时间、乳化温度、搅拌速度、水油相比、明胶浓度等)进行了研究。结果表明,制备微球的最佳工艺条件为:span-80与tween-80以1∶3(质量比)的比例形成复配乳化剂,乳化剂浓度为3.5%,乳化时间为35min,乳化温度为65℃,搅拌速度为900r/min,水油相比为1∶20,明胶溶液浓度0.15g/ml。

Rifampicin microspheres production process is optimized through orthogonal experiment and uni-variation conditions,such as the type of surfactant,the amount of emulsifier,emulsifying temperature,emulsifying time,stirring speed,the proportion of the water phase and the organic phase and the concentration of gelatin.The experimental results show that the optimal conditions are the ratio of span-80and tween-80(1∶3)to form emulsifiers,the amount of emulsifier is 3.5%,emulsifying time is 35min,emulsifying temperature is 65℃,the stirring speed is 900r/min,ratio of water to oil is 1∶20,the gelatin concentration is 0.15g/mL