不同来源人未成熟卵细胞体外成熟及胚胎发育的临床观察

The developmental potentials of immature oocytes collected from different kinds of assisted reproductive technology cycles

目的:比较辅助生殖周期中不同来源未成熟卵细胞体外成熟(IVM)培养后的发育潜能。方法:根据卵细胞来源不同分为IVM组、卵泡减灭组和裸卵组。各组体外培养后的成熟卵细胞分批行夫精卵胞浆内单精子注射(ICSI),统计受精和胚胎发育情况。结果:3组间受精率、卵裂率和优质胚胎率差异无统计学意义(P>0.05)。结论:3种不同来源的未成熟卵细胞经IVM培养均能到达MⅡ期,ICSI受精后可获得形态学上的优质胚胎。

Objective：To compare the developmental potentials of immature oocytes collected from different kinds of assisted reproductive technology （ART） cycles.Methods：in vitro maturation （IVM） group consisted of 25 patients following IVM treatments.Oocytes in follicle aspiration group resulted from follicle fluids collected from 12 patients with in vitro fertilization cycle,who were at risk of developing ovarian hyperstimulation syndrome.Ten patients following intracytoplasmic sperm injection （ICSI） treatment were served as denuded oocyte group.After IVM,matured oocytes were inseminated by ICSI with their husbands＇ sperm.Then the fertilization rate and embryo development were evaluated.Results：There were no statistical differences in the fertilization rate （P ＞0.05）,the cleavage rate （P ＞ 0.05） and the proportion of high-quality embryo （P＞0.05） among three groups.Conclusion：Immature oocytes collected from different kinds of ART cycles could reach M Ⅱ,and high-quality embryo could obtained from the oocyte after fertilization by ICSI.