氨基葡萄糖的柱前衍生化HPLC法测定及其体外透皮吸收

Determination of Glucosamine by Precolumn Derivative HPLC and Its Percutaneous Absorption in vitro

建立了柱前衍生化高效液相色谱法测定氨基葡萄糖,并考察其体外透皮吸收性能.使用丹磺酰氯作为衍生化试剂,采用C18色谱柱,以水∶乙腈（70∶30）为流动相,检测波长250 nm.氨基葡萄糖在10～1 000 μg/ml浓度范围内线性关系良好;回收率为101.8％,RSD为2.41％.采用改良的Franz扩散池,以离体大鼠鼠皮为透皮屏障,测得氨基葡萄糖的透皮速率为42.110 μg·cm-2.h-1,渗透系数为1.610×10-4cm·h-1.

An HPLC method was established for the determination of glucosamine, and the glucosamine permeating through the rat skin in vitro was evaluated. Dansyl chloride was used as derivatization reagent. A C18 column was used with the mobile phase of water ： acetonitrile （70 ： 30） at the detection wavelength of 250 nm. The calibration curve was linear in the concentration range of 10 - 1 000 ug/ml. Its recovery was 101.8%, with RSD of 2.41%. The penetration experiment in vitro with the skin of rat was performed on modified Franz diffusion cells. The percutaneous rate and the permeability coefficient of glucosamine were calculated as 42.110 ug.cm-2.h-1 and 1.610× 10- 4 cm.h-1, respectively.