内蒙古地区牛羊口蹄疫灭活疫苗的免疫效果比较研究

The Research on Comparison of Inactivated Vaccine between Cattle and Sheep with Foot and Mouth Disease in Inner Mongolia

为准确掌握牛羊口蹄疫疫苗免疫效果,2012年对内蒙古自治区的牛羊口蹄疫O-AsiaⅠ型双价灭活疫苗和牛羊口蹄疫A型灭活疫苗免疫效果进行了监测。采用液相阻断ELISA方法,共检测了8个旗县23018份免疫牛羊口蹄疫O-AsiaⅠ型双价灭活疫苗和牛羊口蹄疫A型灭活疫苗15日、30日、60日的血清中口蹄疫免疫抗体水平。结果显示,牛羊口蹄疫疫苗免疫效果总体良好,免疫抗体合格率超过了70%。规模化奶牛养殖场免疫效果稳定,散养地区免疫效果稳定性差,且不同厂家的疫苗免疫效果不同,加强免疫后A、B、D三个厂家抗体合格率能达到100%,C厂家的免疫效果较差,抗体合格率为93.3%。采用3ABC-ELISA检测试剂盒对部分地区牛羊进行感染抗体检测,结果为阴性。

In order to master the immune effect of foot and mouth disease （FMD） vaccine accurately, we monitored the immunity efficacy of foot and mouth disease vaccines used in Inner Mongolia in 2012. We collected 23018 serums of animals from 8 flag counties which had been immunized with strain O and strain Asia I foot and mouth disease inactivated bivalent vaccine 15 clays ,30 days ,60 days ago. The antibody titer was detected by liquid phase blocking ELISA. The results showed that the overall immunity efficacy of foot and mouth disease vaccine was good, and the qualified rate of immune antibody was 70%; the immunity efficacy of large- scale farmers was steady, and it showed poor stability in free-ranging areas. The immunity efficacy of foot and mouth disease vaccines from different manufacturers was different. After booster immunization, the immune antibody of the vaccines from manufacturers A, B and D were completely qualified. The vaccines from manufacturer C showed poorer immune effect, the qualified rate was 93.3%. We conducted antibody detection to cattle and sheep from different areas using 3ABC-ELISA detection kit, the results were negative.