UPLC-MS/MS法检测动物性食品中肝脏、肾脏组织氯霉素残留量

Determination of Chloramphenicol Residues in Liver and Kidney Tissues of Animal Derived Food by UPLC- MS /MS

建立了动物性食品中肝脏、肾脏组织氯霉素残留检测的超高效液相色谱-串联质谱（UPLC-MS／MS）分析方法。样品用β-葡萄糖醛酸苷酶酶解后乙酸乙酯提取，C18（500mg／3cc）固相萃取柱净化，液相色谱-串联质谱负离子模式测定，内标法定量。结果表明：氯霉素在0．5～10．0ng／mL的系列浓度范围内均呈良好线性关系，相关系数r均大于0．990。样品中氯霉素的检测限为0．1μg／kg，定量限为0．2μg/kg。本方法氯霉素在0．2、0．4、2μg／kg的添加浓度范围内，平均回收率均为70％～120％，相对标准偏差（RSD）均小于20％。该方法具有操作简便快捷、结果灵敏度高、准确度高等优点。

Anuhra performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS） method was established for the determination of chloramphenicol in liver and kidney tissues of animal derived food.The sample was hydrolysised by β-glucuronidase enzyme, extracted with ethyl acetate, purified by C18 （500mg/3cc） solid phase extraction and measured by internal standard method with negative ion mode of liquid chromatography- tandem mass spectrometry determination. The calibration curves was good linear between the peak areas and the concentrations of 0.5- 10.0 ng/mL, and the correlation coefficients （r） was more than 0.990. The limit of detection of chloramphenicol was 0.1 μg/kg, and the limit of quantification was 0.2 μg/kg. The average recoveries of chloramphenicol ranged from 70% to 120% . The RSD of method was less than 20%.