东莨菪碱对强迫游泳大鼠雷帕霉素靶蛋白及脑源性神经营养因子表达的影响

Effects of scopolamine on the expression of hippocampal mammalian target of rapamycin and brain-derived neurotrophic factor of rats in the forced swimming test

目的探讨东莨菪碱对强迫游泳大鼠海马雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)及脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)表达的影响。方法雄性Wistar大鼠20只,随机均分为两组(n=10):对照组(C组)和东莨菪碱组(S组),行强迫游泳15 min建立大鼠抑郁模型。次日,分别腹腔注射等容积生理盐水和东莨菪碱0.04 mg/kg。给药后60 min再次行强迫游泳实验5 min,观察并记录不动时间。行为学测试后,取海马组织测定mTOR和BDNF的含量。结果与C组相比,S组大鼠强迫游泳不动时间显著减少,海马组织中mTOR及BDNF的表达显著增高。结论东莨菪碱的抗抑郁作用可能与海马组织中mTOR及BDNF的上调有关。

Objective To investigate the effects of scopolamine on the expression of hippocampal mammalian target of rapamycin and brain -derived neurotrophic factor of rats in the forced swimming test . Methods Twenty male Wistar rats were randomly divided intocontrol group （ C group,n=10） and scopolamine group （Sgroup,n=10）.Rats were forced to swimming for 15 min to construct depression animal model .On the second day, rats in group C and S were intraperitoneal injected with the same volume of saline and 0.04 mg/kg scopolamine .Sixty minutes later , rats were forced to swim again for 5 min, record the immobility time .Detect the expression of mTOR and BDNF in hippocampus after behavioral test .Results Compared with C group ,immobility time of rats in S group decreased significantly during forced swimming test , and the expression of mTOR and BNDF in hippocampusincreased significantly .Conclusions The antidepressant effect of scopolamine might related to the up-regulation of mTOR and BDNF in hippocampus .