血管生成素2在食管鳞状细胞癌中的表达及其意义

Expression of angiopoietin-2 in esophageal squamous cell carcinoma and its significance

目的 分析血管生成素2（Ang-2）在食管鳞状细胞癌中的表达,并探讨其与患者临床病理特征之间的关系.方法 采用免疫组织化学PV-9000非生物素二步检测方法,用Ang-2单克隆抗体对60例食管鳞状细胞癌手术切除标本、5例癌旁正常食管上皮和30例正常食管上皮的石蜡标本组织切片进行染色,并观察、评定Ang-2蛋白表达的阳性率.分析Ang-2的表达与标本临床病理指标的关系.结果 Ang-2阳性产物主要定位于细胞质.正常食管上皮、食管癌旁组织、食管鳞状细胞癌中Ang-2的阳性表达率分别为3.33％（1/30）、40.00％（2/5）、53.33％（32/60）,与正常食管组织相比,食管鳞状细胞癌Ang-2阳性表达明显升高,差异具有统计学意义（P＜0.05）;食管鳞状细胞癌中,Ang-2表达与浸润深度呈正相关（rs=0.621,P=0.002）;与分化程度呈负相关（rs=-0.309,P=0.018）.结论 Ang-2可能在食管鳞状细胞癌的发生、发展中起重要作用.肿瘤分化程度、浸润深度以及TNM分期是食管鳞状细胞癌的独立预后指标,检测Ang-2表达对食管鳞状细胞癌的早期诊断、预后判断有重要参考价值.

Objective To analyze expression of angiopoietin-2 （Ang-2） protein in human esophageal squamous cell carcinoma （ESCC）, and to investigate the relationship between Ang-2 protein and clinicopathologic factors. Methods 60 cases of ESCC, 5 cases of latero-carcinoma tissues and 30 cases of normal tissue were immunohistochemically stained using Ang-2 monoclonal antibodies with PV-9000 non-biotin detection system. The positive expression rate and expression level of Ang-2 were evaluated. Results Ang-2 was mainly expressed in cytoplasm. The positive expression rates in normal epithelium, latero-careinoma tissues and ESCC were respectively 3.33 % （1/30）, 40.00 % （2/5）, 53.33 % （32/60）. Compared ESCC with normal epithelium and latero-eareinoma tissues, Ang-2 positive expression rate was significantly higher （P 〈 0.05）. Among ESCC, expression of Ang-2 was positively correlated with the tumor infiltration depth （rs=- 0.621, P = 0.002）, and inversely correlated with tumor differentiation status （r = -0.309, P -- 0.018）. Conclusions Ang-2 may play an important role in the carcinogenesis and development of ESCC. Tumor differentiation degree, infiltration depth and TN＇M staging are the independent prognostic indicators for ESCC patients. Combinative detecting the expression of Ang-2 may provide more accurate information in early diagnosis and predicting the prognosis of ESCC.