重离子束辐照对绵羊卵母细胞及体外受精卵早期发育的影响

Effects of Heavy Ion Radiation on Oocytes and Early Development of In Vitro Fertilization Eggs in Sheep

试验探索重离子束辐照对绵羊卵母细胞的体外成熟及体外受精卵早期胚胎发育的影响。用不同剂量的重离子束辐照绵羊卵母细胞和精液,观察卵母细胞体外成熟率及体外受精后早起胚胎的卵裂率、桑椹胚率及囊胚率。试验结果显示:与对照组相比,1.0、1.5Gy辐射可以明显提高绵羊卵母细胞的体外成熟率(p<0.01);重离子辐照绵羊精液和卵母细胞并做体外受精后,早期胚胎发育有着显著变化。当用0.5、1.0Gy剂量辐照精液时,绵羊早期胚胎的卵裂率和桑葚胚率显著升高(p<0.05),囊胚率无显著变化,1.5 Gy剂量,卵裂率显著提高(p<0.05),2.0 Gy剂量,卵裂率、桑葚胚率、囊胚发育率均极显著降低(p<0.01);当重离子束辐照卵母细胞时,0.5、1.0Gy剂量组的卵裂率、桑葚胚率和囊胚发育率显著升高(p<0.05),而2.0Gy剂量组的卵裂率和桑葚胚发育率极显著降低(p<0.01),而囊胚发育率为0。结果表明低剂量的重离子辐照绵羊精液、卵母细胞,对体外受精和早期胚胎发育具有明显的促进作用,而高剂量辐照则抑制细胞的生长发育。

The current experiment was conducted to examine the effect of heavy ion radiation in vitro maturation of oocytes and early development of fertilization eggs of sheep. Using different doses of heavy ion irradiation on oocyte and semen, we observed the ratio of in vitro maturation of oocytes and cleavage morula blastocyst of early embryos after fertilization. The results show that 1.0 and1.5 Gy could improve significantly the ratio of in vitro oocyte maturation compared with control group（p〈0.01）; early development of the embryo had a significant change after fertilization of semen and oocyte by heavy ion radiating. When irradiatied on semen, the ratio of cleavage and mulberry embryo of early embryos increased significantly by 0.5and1.0 Gy dose irradiating（p〈0.05）, but the ratio of blastocyst had no significant change. After radiating by1.5 Gy dose, the ratio of cleavage was significantly increased（p〈0.05）, while the cleavage, mulberry embryo and blastocyst was significantly decreased when radiation was 2.0 Gy dose（p〈0.01）. When irradiatied on oocyte, the ratio of cleavage,mulberry and blastocyst increased significantly when radiation were 0.5and1.0 Gy dose irradiating（p〈0.05）,and the ratio of cleavage and mulberry significantly reduced （p〈0.01） after irradiating by 2.0 Gy, but the ratio of blastula development is zero after oocyte radiated by 2.0 Gy dose. Our finding suggested that low doses of heavy ion radiation promoted in vitro fertilization obviously, the early embryonic development but high doses of radiation inhibited cell growth.