雷公藤药材中雷公藤甲素及总二萜内酯的含量测定

Dedtermination the Content of Triptolide and Total Diterpene Lactones in Tripterygium Wilfordii Hook.F.

目的 建立雷公藤药材中雷公藤甲素及总二萜内酯含量测定的方法.方法 采用高效液相色谱法,色谱柱：Platisil ODS柱（250mm×4.6mm,5μm）,以甲醇-水（45∶55）为流动相进行洗脱,于218nnm波长处检测,测定雷公藤甲素的含量;采用Kedde显色法,在550nm波长处测定吸光度,测定总二萜内酯的含量.结果 以高效液相色谱法测定雷公藤甲素,在0.58～46.40μg·mL-1（r=0.9999）范围内线性关系良好,平均回收率为100.52％ （n =6）;比色法测定雷公藤总二萜内酯（以雷公藤甲素计）在0.49-39.36μg·mL-1（r =0.9999）范围内线性关系良好,平均回收率为93.28％（n=6）;雷公藤药材中雷公藤甲素平均含量为4.20μg·g-1,总二萜内酯为86.64μg·g-1.结论 本实验所建立的方法稳定,精密度良好,适合于雷公藤甲素及总二萜内酯的含量测定,可用于雷公藤药材的质量控制.

Objective To develop methods for the determination of total diterpene lactones and triptolide in Tripterygium wilfordii Hook F. Methods The HPLC analysis for triptolide was carried on an Platisil ODS column （250mm x 4. 6mm, 5ktm）, to the methanol： water（45 ： 55） as mobile phase elution and UV detection at 218nm ,determine the content of triptolide. A colorimetric method was established for the assay of the total diterpene lactones with 3,5-dinitrobenzoic acid as the coloring reagent and measuring of the absorbance at 550nm. Results The calibration curves were linear in the ranges from 0. 58-46. 40μg ·mL^-1（ r =0. 9999） for triptolide with HPLC and from 0.49-39. 36μg ·mL^-1 （ r = 0. 9999） for the total diterpene lactones （caculated as triptolide） with colorimetrie method. The average recoveries（n = 6） were 100. 52 % and 93.28 %, re- spcctively. The average content of triptolide in Tripterygium wilfordii Hook. F. is 4. 20μg ·mL^-1, total diterpene lactones is 86. 64μg ·mL^-1. Conclusion The method is in good reproducibility, precision and stability and is fit to determine the total diterpene lactones and triptolide in Tripterygium wil- fordii Hook. K At the same time , they can be used for the assay and quality control of the Tripterygium wilfordii Hook. F.