七氟醚预先给药对大鼠肾缺血再灌注时炎性反应的影响

Effect of sevoflurane pretreatment on inflammatory response during renal ischemia-reperfusion in rats

目的 评价七氟醚预先给药对大鼠肾缺血再灌注时炎性反应的影响.方法 清洁级健康雄性SD大鼠30只,12～ 14周龄,体重220 ～ 260 g,采用随机数字表法,将其分为3组（n=10）：假手术组（S组）、肾缺血再灌注组（I/R组）和七氟醚预先给药组（SP组）.I/R组和SP组采用夹闭左肾蒂45min后恢复灌注的方法制备大鼠肾缺血再灌注损伤模型,S组腹部正中切口,右肾切除,左肾蒂游离后,缝合腹腔；SP组模型制备前30 min开始吸入3％七氟醚和氧气的混合气体.于再灌注3h时采集下腔静脉血样5 ml,测定血清BUN和Cr的浓度,光镜下观察肾组织病理学结果,测定肾组织TNF-α、IL-6和细胞间黏附分子-1（ICAM-1）的含量.结果 与S组比较,I/R组和SP组血清BUN和Cr浓度、肾脏近曲小管坏死程度分级、肾组织TNF-α、IL-6和ICAM-1含量升高（P＜0.05）；与I/R组比较,SP组血清BUN和Cr浓度、肾脏近曲小管坏死程度分级、肾组织TNF-α、IL-6和ICAM-1含量降低（P＜0.05）.结论 七氟醚预先给药通过抑制肾组织炎性反应减轻大鼠肾缺血再灌注损伤.

Objective To evaluate the effect of sevoflurane pretreatment on the inflammatory response during renal ischemia-reperfusion （I/R） in rats.Methods Thirty pathogen-free male Sprague-Dawley rats,aged 12-14 weeks,weighing 220-260 g,were randomized into 3 groups （n =10 each） using a random number table：sham operation group （group S）,I/R group and sevoflurane pretreatment group （group SP）.Renal I/R was induced by clamping the left renal pedicle for 45 min followed by reperfusion in I/R and SP groups.In group S inhalation of 3% sevoflurane in O2 was started at 30 min before I/R and maintained throughout the experiment.Venous blood samples were taken at 3 h of reperfusion for determination of serum blood urea nitrogen （BUN） and creatinine （Cr） concentrations.The animals were then sacrificed and the left kidneys were removed for microscopic examination and for measurement of the content of tumor necrosis factor-apha （TNF-α）,interleukin-6 （IL-6） and intercellular cell adhesion molecule-1 （ICAM-1） in renal tissues （by ELISA）.Results Compared with group S,the serum BUN and Cr concentrations,severity of necrosis of renal proximal convoluted tubules （0 =normal,4 =necrosis of whole segment of proximal convoluted tubules）,and contents of TNF-α,IL-6 and ICAM-1 were significantly increased in I/R and SP groups （P 〈 0.05）.Compared with group I/R,the serum BUN and Cr concentrations,severity of necrosis of renal proximal convoluted tubules,and contents of TNF-α,IL-6 and ICAM-1 were significantly decreased in SP group （P 〈 0.05）.Conclusion Sevoflurane pretreatment can protect kidney against I/R injury by inhibiting the inflammatory responses in the renal tissues of rats.