应用响应面法优化黑曲霉固态发酵稻谷壳产木聚糖酶条件

Study on optimization of the rice crust solid-state fermentation for xylanase from Aspergillus niger by response surface method

对黑曲霉产木聚糖酶发酵工艺进行优化研究。在单因素实验的基础上,采用Box-Benhnken实验设计,对黑曲霉发酵稻谷壳产木聚糖酶的最佳碳源及其添加量、培养时间、水的添加量进行优化,建立了木聚糖酶产量随蔗糖添加量、培养时间和水的添加量变化的多项二次回归方程,并得到最佳的发酵工艺条件是:培养时间为78h,水的添加量为57%,蔗糖添加量为1.3g时,木聚糖酶的酶活力可达221.5U/g,比优化前提高了1.76倍,预测模型可靠,可利用该模型对黑曲霉产木聚糖酶的最优发酵条件进行理论预测。

In this study,the fermentation technology of xylanase produced by Aspergillus niger in solid-state fermentation using rice crust as a sole substrate was investigated. On the basis of single factor experiment,the best carbon source,fermentation time and content of water for xylanase production by Aspergillus niger were optimized by Box-Benhnken design,and a quadratic regression equation for the change of enzymic activity with soluble starch,fermentation time,the content of water was established. The results showed that the optimal conditions were fermentation time for 78h,the content of water 57%,soluble starch 1.3g. Under optimization conditions,xylanase activity reached 221.5U/g,increased 1.76 times than before optimization.The prediction model indicated high reliability,which could be applied to optimization of xylanase fermentation conditions.