摘要
研究了白簕叶黄酮的提取纯化及抗炎效果。实验采用正交设计法优化了超声辅助提取法提取白簕叶黄酮,并考察了大孔树脂的纯化效果。采用50只SD大鼠,分为阴性对照组、地塞米松阳性对照组、白簕叶黄酮低、中、高剂量组,灌胃给药7d,末次给药后1h,分别测定大鼠足肿胀度,考察各组用药对角叉菜胶致大鼠足趾肿胀的影响。结果显示,采用最优方案提取白簕叶黄酮并进行纯化,所得提取液中总黄酮含量为37.01mg/g,纯化后黄酮纯度达到24.36%。将SD大鼠连续给药7d后,白簕黄酮提取物10、20、40mg/(kg·d)均能有效抑制角叉菜胶致大鼠足趾肿胀程度。研究结果表明,白簕叶黄酮具有抗炎效果,进一步开发了白簕的药用价值。
To study the extraction,purification and anti-inflammatory effect of total flavonoids from Acanthopananx Trifoliatus（L.） Merr. leaves,the orthogonal test was carried out to optimize the ultrasonic-assisted extraction method. 50 rats were divided into normal group,negative control group,positive control group,flavonoid lowdose group,flavonoid mid-dose group and flavonoid high-dose group. 7d after dosing by intra-peritoneal injection,carrageenan-induced hind paw edema in rats were conducted to investigate the anti-inflammatory effect. The content of total flavonoids in extraction with the optimized protocol was 37.01mg/g,and purity was up to 24.36% after purification. Total flavonoids at dose of 10,20,40mg/（kg·d） could inhibite swelling induced by carrageenan. Results showed that total flavonoids from Acanthopanax trifoliatus（Linn.） Merr. leaves had anti-inflammatory effect,which further develop the medicinal value of Acanthopanax Trifoliatus（Linn.） Merr.
出处
《食品工业科技》
CAS
CSCD
北大核心
2014年第8期295-298,共4页
Science and Technology of Food Industry
关键词
白簕
总黄酮
超声辅助提取法
大孔树脂纯化
抗炎
Acanthopanax trifoliatus（Linn.） Merr.
total flavonoids
ultrasonic-assisted extraction
macroporous adsorption resins
anti-inflammatory effect
