高效提取桑葚多糖的工艺优化研究

Efficient Extraction and Process Optimization of Fructus Mori Polysaccharide by HPEF

研究利用高压脉冲电场(HPEF)对桑葚进行破壁提取桑葚多糖,并采用分光光度法测定桑葚中的多糖含量。通过对HPEF提取桑葚多糖工艺的优化,考察了HPEF处理强度、温度、液料比、脉冲频率4因素对多糖含量得率的影响。在单因素试验结果的基础上,进行正交试验设计,试验结果表明:最佳测定条件为A2B2C2D2,即HPEF处理强度为40 kV/cm、HPEF处理温度为90℃、液料比为12︰1(mL/g)、HPEF脉冲频率为12 000 Hz。该法快速、经济、准确,可用于桑葚多糖的高效提取,并得到最佳提取工艺下的得率为15.05%。

In order to obtain the optimization extract of fi＇uctus moil polysaccharides, this research measures polysaccharide content in fructus mori by UV-V is spectrophotometer, investigates the efficient extraction of ffuctus mori polysaccharides under high intensity pulsed electric fields （HPEF） and process optimization, and studies the influences of following 4 factors on the yield ofpolysaccharides： pulse electric filed intensity, temperature, solid-liquid ratio, and pulse frequency. On the basis of single factor experiment, optimize them by the oahogonal experiment. Results indicate that the largest yield of polysaccharides is 15.05% under the optimum extraction condition A2B2C2D2, which is pulse electric field intensity 40 kV/cm, temperature of fixed HPEF 90 ℃, solid-liquid ratio 12 ： 1 （mL/g）, and pulse frequency 12 000 Hz. This method has shown the advantages of fast, sensitive and accurate extraction, which can be used in efficient extraction of FRUCTUS MORI polysaccharides.