盐酸或氨基磺酸催化水解斯替夫苷制备甜菊醇

Preparation of Steviol Through the Hydrolysis of Stevioside Catalyzed by Hydrochloric Acid or Sulfamic Acid

分别以盐酸或氨基磺酸为催化剂水解斯替夫苷(St),研究底物质量浓度、反应温度、催化剂用量和反应时间对斯替夫苷水解的影响,着重考察了斯替夫苷的转化率、甜菊醇的产率和异甜菊醇的产率。其中,盐酸催化水解斯替夫苷的最优工艺条件为:反应温度95℃,底物质量浓度400 g/L,盐酸浓度0.04 mol/L,反应时间28 h;在该条件下,根据高效液相色谱分析计算得到斯替夫苷转化率接近100%,甜菊醇产率达83.6%,异甜菊醇产率达12.4%。氨基磺酸催化反应的工艺条件为:反应温度95℃,斯替夫苷质量浓度400 g/L,氨基磺酸浓度0.05 mol/L,反应时间31 h;在该条件下,根据高效液相色谱分析计算得到斯替夫苷转化率接近100%,甜菊醇产率达81.5%,异甜菊醇产率达11.7%。

Hydrochloric acid and sulfamic acid were respectively applied to catalyze the hydrolysis of stevioside （St）. The effect of the factors of temperature, St concentration, catalyst amount and reaction time on the hydrolysis of stevioside catalyzed by hydrochloric acid or sulfamic acid was investigated. The results show that,when hydrochloric acid was used as catalyst, stevioside conversion was nearly 100%, the yield of steviol reached 83.6% and the yield of isosteviol reached 12. 4% under the optimum conditions. With the catalyst of sulfamic acid,a conversion of stevioside of nearly 100% ,a yield of steviol of 81.5% and a yield of isosteviol of 11.7% were obtained under the optimum conditions.