摘要
建立了高效液相色谱法测定慕萨莱斯中儿茶素含量。采有ZORBAX80AExtend—C18（4．6mm×150mm，5um）；流动相：坎甲醇）：V（1％乙酸水溶液（pH3））=10：90；流速：1．0mL／min；柱温：30℃；进样量：20uL：检测波长：280nm。儿茶素进样量在0．02-1．6ug范围内与峰面积具有良好的线性关系。该方法的儿茶素最低检出限为1．46wg／kg。儿茶素平均回收率为99．8%，KSD为0．38％。
To establish method of reversed phase hi-gh-performance liquid chromatography to determine the content of catechin in Musalas. RP-HPI_C was adopted, on a column of ZORBAX 80A Extend-Ct8 （4.6 ram× 150 mm, 5 Ima）; Mobile phase was methanol ： 1% aqueous acetic acid （pH 3） =10 ： 90; At a flow rate of 1.0 mlJmin, column temperature was 30 ℃; Injection volume was 20 p.L and the detection wavelength of 280 nm. In the range of 0.02-1.6 Ixg, the catechin injection volume and the peak area having good linear relationship. The lowest detection limit of catechin was 1.46 ixg/kg. The average recovery rate was 99.8%, and RSD was 0.38%. The method is accurate, simple and sensible, and is suitable for the determination ofcatechin content in Musalas.
出处
《食品工业》
北大核心
2014年第4期204-206,共3页
The Food Industry
关键词
慕萨莱斯
高效液相色谱法
儿茶素
Musalas
high-performance liquid chromatography
catechin
