籽瓜瓤多糖的单糖组分测定及抗氧化活性评价

Study on the Monosaccharide Composition and Antioxidant Activity of Polysaccharide in Seed-watermelon Flesh

籽瓜瓤经水提醇沉法提取籽瓜瓤多糖,洗脱得一个多糖组分,采用高效离子色谱法测定了籽瓜瓤多糖的单糖组分为鼠李糖、阿拉伯糖、半乳糖、葡萄糖、木糖、半乳糖醛酸、葡萄糖醛酸;籽瓜瓤多糖的抗氧化活性表明籽瓜瓤多糖对·OH的清除率最大为28.91%,对MDA的抑制率最大为10.46%;对·O2-的清除率最大为15.17%。相同浓度条件下,籽瓜瓤多糖具有一定的抗氧化活性,但对·O2-和·OH清除率以及对MDA的抑制率均比VC对三者的清除率或抑制率小。

The crude polysaccharide was fractioned by Sevage law to take off the protein, then separated and dialyzed by Sephadex G-75 gel filtration. The monosaccharide components can be obtained by efficient ion chromatography from polysac- charide component, which are： rharrmose, ambinose, galactose, glucose, xylose, galacturonic acid and glucuronic acid. The antioxidant activity results of extracting polysaccharide isolation from melon seeds matures showed that the most clearance rates for .OH of the melon seed matures polysaeeharide was 28.91%, meanwhile the maximum inhibition rates for MDA was 10.46%, and the most clearance rates for ＂02- was 15.17%. In the same concentration conditions, the clearance rates for ＂OH and ＂02 and the inhibition rotes for DNAs of the melon seed matures polysaccharides were lower than VC.