以玉米浆和木薯为原料机械搅拌发酵制备细菌纤维素的研究

Production of bacterial cellulose from corn steep liquor and cassava hydrolysates in mechanical agitating fermenter

本文以玉米浆和木薯为原料,用机械搅拌式发酵罐制备细菌纤维素(BC),对发酵过程的纤维素产量、还原糖消耗、溶氧变化和茵浓变化进行了监测,并以葡萄糖-蛋白胨一酵母粉培养基为对照进行了比较。实验得出玉米浆作氮源时不溶BC的产量为9.2 g/L,而氮源成本只是对照组的15%;木薯水解液作碳源时的不溶BC产量达到11.7 g/L,比对照组(10.8 g/L)高8%;而用玉米浆搭配木薯水解液发酵生产BC,产量也达到10.1 g/L,验证了这两种天然原料的廉价高效性,用于工业生产细菌纤维素具有良好的前景。

In this paper corn steep liquor （CSL） and enzymatic hydrolysates of cassava were used as feedstocks to produce bacterial cellulose （BC） in an aerated and agitated fermenter. The yield of BC, residual sugar, dissolved oxygen and bacterial live cells were determined and compared with the result from a glucose-peptone-yeast extract （ G ＋ P ＋ Y） culture medium, which was used as a control. When glucose-CSL was used, the yield of BC reached 9.2 g/L but the cost of the nitrogen source CSL was only 15% of the control. When cassava hydrolysates-peptone-yeast extract was used, the yield of BC reached 11.7 g/L, which was 8% higher than that of the control （10.8 g/L）. When both CSL and cassava hydrolysates were used, the yield of BC reached 10.1 g/L. Due to their high performance but low cost, CSL and cassava could be widely used in industrial production of BC in future.