T淋巴细胞亚群、自然杀伤细胞及B淋巴细胞的变化对肝癌家族聚集性影响的研究

Impact of changes of T lymphocyte subpopulation,natural killer cells and B lymphocytes on familial aggregation of liver cancer

目的通过对肝癌高发家族成员外周血T淋巴细胞亚群、NK细胞及B淋巴细胞的检测,探讨机体免疫状态在肝癌发生及家族聚集中的作用,为临床治疗提供参考依据。方法收集2008年3月-2013年3月以配对方法选取广西肝癌高发区的33个肝癌高发家族及59个无癌家族成员作为研究对象;利用流式细胞术检测两组成员外周血T淋巴细胞亚群、NK细胞、B细胞的频数表达,采用SPSS16.0统计软件进行数据分析。结果与无癌家族相比,肝癌高发家族成员外周血CD3+、CD4+T淋巴细胞、CD4+/CD8+比值、NK细胞明显降低;CD8+T淋巴细胞和B淋巴细胞明显升高;分层分析发现肝癌高发家族成员中肝癌患者、HBsAg阳性的家族成员的CD4+T淋巴细胞、CD4+/CD8+比值、NK细胞均低于对照组,而未患肝癌的高发家族成员和HBsAg阴性的高发家族成员的CD4+T淋巴细胞、CD4+/CD8+比值、NK细胞也低于对照组。结论肝癌高发家族成员存在细胞免疫功能低下,促进肝癌的发生并呈家庭聚集,这些变化在肝癌发生前已存在,可能为遗传因素引起。

OBJECTIVE To investigate the effect of organism immune state on the occurrence of liver cancer and family aggregation according to the detection of peripheral blood T lymphocyte subpopulation, NK cells and B lymphocytes, so as to provide reference basis for clinical data. METHODS Selected 33 families with high liver cancer incidence and 59 families without liver cancer members as the object of study during Mar 2008 to Mar 2013. The frequencies of peripheral blood T lymphocyte subpopulation, NK cells and B lymphocytes was cletected by flow cytometry （FCM）, and the data analyzed were with SPSS 16.0 software. RESULTS Compared with the fami- lies without liver cancer members, the number of peripheral blood CD3＋, CD4＋ T cells, CD4 ＋/CD8 ＋ ratio and NK cells of the families with high liver cancer incidence decreased significantly; according to the stratified analysis, the number of CD4 ＋ T lymphocyte and NK cell as well as the CD4 ＋/CD8 ＋ ratio in the family members who had liver cancer and positive HBsAg were obviously lower than those of the control group, while in the family members with high liver cancer incidence who had no liver cancer but had positive HBsAg, the same figures were lower than those of the control group. CONCLUSION HCC high-occurrence family members have low cellular immunity, which is easy to cause liver cancer featuring family aggregation; all these changes have been existed before suffer- ing from liver cancer, which may be caused by generic factor.