正畸力作用下大鼠牙髓组织中热休克蛋白70的表达及意义

Expression of HSP70 in rat molars pulp tissue applied with orthodontic force

目的检测正畸力作用下大鼠牙髓组织中热休克蛋白70(Hsp70)的表达和分布。探讨其在正畸移动中的作用和机制。方法建立大鼠正畸牙移动模型,分别在受力1、15、30d处死,制备左侧上颌第一磨牙及其周围牙槽骨的石蜡标本,采用免疫组织化学法检测大鼠正畸牙移动过程中牙髓组织中Hsp70的表达情况。结果正常对照组、正畸加力1d组、正畸加力15d组和正畸加力30d组牙髓组织中Hsp70表达量分别为4.02±0.03、28.13±0.23、10.02±0.08、5.23±0.42,正畸加力1d组大白鼠牙髓组织中Hsp70表达量显著高于正常对照组(P<0.01),正畸加力15d组和正畸加力30d组与正常对照组的差异无统计学意义(P>0.05)。结论 Hsp70参与了正畸牙移动,并且保护了牙髓组织。

Objective To investigate the expression of heat shock protein 70 （Hsp70） in rat molars pulp applied with or- thodontic force. Methods The animal model for tooth movement was established, and the rats were sacrificed in batches on dl, 15 and 30. The maxillary molars and periodontium specimens were obtained and the expression of Hsp70 was detected by im- munohistochemistry. Results The expression of Hsp 70 in control group and dl, d15, d30 orthodontic force groups were 4.02± 0.03 and 28.13 ±0.23, 10.02 ±0.08, 5.23 ±0.42, respectively. There was significant difference in Hsp 70 expression between control group and D1 orthodontic force group （P〈0.01）, while there were no significant differences between control group and D15, D30 groups （P 〉0.05）. Conclusion During the orthodontic tooth movement, HSP70 might be one of main cytokines in pro- cess of reparative dentin mineralization.