伴性赤蚁基因(sch)的RAPD分子标记研究

STUDIES ON THE MOLECULAR MARKER OF THE SEX-LINKED CHOCOLATE GENE(sch )IN SILKWORM WITH RAPD

用夏芳♂、夏ｓｃｈ♂、秋白♂、秋ｓｃｈ♂两对近等位基因系为材料，采用随机引物进行 ＰＣＲ扩增。通过９０个随机引物，在夏ｓｃｈ♂中得到ＯＰＤ０２－４００ｂｐ多态性特异带，用秋白、秋ｓｃｈ进 一步验证确认：ＯＰＤ０２－４００ｂＰ是ｓｃｈ基因的 ＲＡＰＤ标记。将获得的分子标记 ＤＮＡ片段回收纯化 后，克隆到质粒ｐＢｌｕｅｓｃｒｉｐｔ－ＩＩＳＫ载体上。

PCR amplification was made with arbitrary primers, using two pairs of near - isogenic lines(Xiafang ♂ and Xia sch ♂, and Qiubai ♂ and Qiu sch ♂ ) of silkworm as the experiment materials. A polymor- phic specific band, OPD02 - 400bp, was obtained with 90 arbitrary primers in Xia Sch ♂. A further ex- periment with Qiubai and Qiu sch confirmed that OPD02 - 400bp is the PAPD marker for the sex - linked chocolate gene sch. The DNA fragments of the molecular marker were recovered, purified and cloned onto the carrier - plasmid pBluescript - II SK.